GENETICS AND GENOME BANKING CORE

遗传学和基因组库核心

• 批准号: 8358105
• 负责人: Hans Michael Kubisch
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358105
• 关键词: Alleles; Animals; Biopsy; Blood; Blood specimen; Breeding; Cell Line; DNA; DNA Library; Data; Derivation procedure; Development; Equilibrium; Equipment and supply inventories; Fibroblasts; Funding; Gene Frequency; Generations; Genetic; Genome; Grant; Infant; Methods; Microsatellite Repeats; National Center for Research Resources; Outcome; Parents; Partner in relationship; Primates; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sampling; Short Tandem Repeat; Source; Testing; Tissues; United States National Institutes of Health; Universities; age effect; cost; father role; genetic resource; male; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Introduction: The efforts of the Core have focused on banking of genetic resources and on generation of microsatellites to assign paternity and determine allele frequencies. Methods: Blood and tissue biopsies were collected during routine inventories. Blood was used for DNA extractions and for banking using archival blood cards. Biopsies were processed to establish and cryopreserve fibroblast cell lines. Results/Discussion: During the past year the Core obtained blood samples from 734 animals which were blotted on archival blood cards. The current number of blood samples banked on cards is 5169. The Core also obtained 302 tissue biopsies from which a total of 193 fibroblasts cultured were generated and cryopreserved. Analysis of the outcome of fibroblast derivations revealed a significant age effect with biopsies from older animals yielding fewer cell lines. The number of cell lines banked to date is 329. Short tandem repeats (STRs) were generated from 281 animals and were sent off to the University of Davis (UD) for allele determination. This included samples from 78 SPF4 potential breeding males. To date, results were obtained from UD for 190 animals. Analysis of allele data from these potential breeders revealed that the overall degree of heterozygosity at all loci deviates from what would be expected under equilibrium conditions such as completely random mating (observed: 0.62, expected: 0.72, p=0.006). Paternity and/or maternity were assigned to 36 infants, most of which were part of the SPF9 colony. We do not yet have STR profiles on file for all parents and final parentage determinations will be done when those have arrived. The Core participated in activities of the NPRC Consortium Genome Banking and Genetics Working Groups in the establishment of a DNA bank and the development and testing of SNP panels for paternity an ancestry analysis.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 简介：核心小组的工作重点是建立遗传资源库，并制作微卫星，以确定亲子关系和等位基因频率。 方法：在常规库存期间收集血液和组织活检。血液用于DNA提取和使用档案血卡进行储存。 处理活检以建立和冷冻保存成纤维细胞系。 结果/讨论：在过去的一年中，中心从734只动物中采集了血样，并将其记录在存档血卡上。目前储存在卡片上的血液样本数量为5169。 Core还获得了302份组织活检，从中产生了总共193个培养的成纤维细胞并冷冻保存。对成纤维细胞衍生结果的分析显示，年龄较大动物的活检产生的细胞系较少，具有显著的年龄效应。迄今为止储存的细胞系数量为329。 从281只动物中产生短串联重复序列（STR），并将其送到戴维斯大学（UD）进行等位基因测定。这包括来自78只SPF 4潜在育种雄性的样本。迄今为止，从UD获得了190只动物的结果。 从这些潜在的育种者的等位基因数据的分析表明，在所有基因座的杂合性的整体程度偏离了什么将是在平衡条件下，如完全随机交配（观察：0.62，预期：0.72，p=0.006）的预期。父亲和/或母亲被分配给36个婴儿，其中大多数是SPF 9群体的一部分。我们还没有所有父母的STR档案，最终的亲子关系确定将在这些档案到达后进行。核心参加了NPRC联合会基因组库和遗传学工作组的活动，建立了DNA库，并开发和测试了用于亲子关系和祖先分析的SNP小组。