MOUSE BRAIN GLYCOPROTEINS EXPRESSING O-MAN AND ASN-LINKED GLYCANS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 一种相对较新的O-连接多糖与神经细胞的黏附和迁移有关，它是O-连接的 甘露糖。该项目将使用新技术从大脑中分离表达O-Man多糖的糖蛋白 组织，并使用质谱学技术鉴定这些糖蛋白。此外，天冬氨酸连接的多糖来自 用MALDI-TOF MS鉴定了小鼠的大脑，并将其与在小鼠体内发现的不含多糖的多糖进行了比较 GnT-Va糖基转移酶，以确定该酶如何调节糖蛋白多糖的表达。A GnT-VB为空 现在已经产生了小鼠，这种缺失对O-Man和ASN连接的糖蛋白的影响现在正在研究中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.