MOUSE BRAIN GLYCOPROTEINS EXPRESSING O-MAN AND ASN-LINKED GLYCANS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 一种相对新型的O-连接聚糖与神经细胞粘附和迁移有关， 甘露糖聚糖 该项目将使用新技术从脑中分离表达O-Man聚糖的糖蛋白 组织并使用质谱技术鉴定这些糖蛋白。此外，来自 小鼠大脑已经通过MALDI-TOF MS鉴定。这些正在与小鼠中发现的聚糖进行比较， GnT-Va糖基转移酶，以确定该酶如何调节糖蛋白聚糖表达。 GnT-Vb空值 现在已经产生了小鼠，这种缺失对O-Man和Asn连接的糖蛋白的影响正在研究中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.