BREAST CARCINOMA GLYCOPROTEIN CHANGES CAUSED BY ONCOGENE EXPRESSION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 人类癌基因HER-2的表达导致N-乙酰氨基葡萄糖转移酶T-V(GlcNAc T-V)选择性增加 信使核糖核酸和活性。使用与GlcNAc T-V产物结合的植物凝集素对乳腺组织进行的组织学研究显示 该产物在未患病的乳腺组织中不存在，但在细胞出现肿瘤时表达。GlcNAc T-V 在肿瘤进展过程中，产物的表达增加。这个项目的重点是识别特定的糖蛋白。 表达GlcNAc T-V产物的乳腺活检组织，以确定可能的乳腺生化肿瘤标记物 癌症。为了鉴定这些糖蛋白，二维等电聚焦/SDS-PAGE技术和LC-Q-TOF 技术正在被使用。PVDF膜经分离和电印迹后，用标记的植物凝集素 鉴定斑点糖蛋白上独特的糖基化模式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Expression of the human oncogene, her-2, causes selective increases in N-acetylglucosaminyltransferase T-V (GlcNAc T-V) mRNA and activity. Histological studies of breast tissue using a plant lectin that binds to the product of GlcNAc T-V show that the product is absent from non-diseased breast tissue, but is expressed when cells appear neoplastic. The GlcNAc T-V product increases in expression during tumor progression. This project focuses on identifying particular glycoproteins in breast biopsy tissue that express the GlcNAc T-V product in order to define possible biochemical tumor markers for breast carcinoma. To identify these glycoproteins, two-dimensional isoelectric focusing/SDS-PAGE techniques and LC-Q-TOF techniques are being used. After separation and electroblotting to PVDF membranes, labeled plant lectins are used to identify unique glycosylation patterns on the blotted glycoproteins.