CORE--Analytical

分析核心为爱荷华大学研究提供常规和非常规分析服务 程序。该核心将提供符合五个目标的专家人员、设备和仪器。具体目标1 是制定分析和质量控制/保证标准。与 合成核心，负责获取和归档单个化合物标准并准备 股票解决方案，分析核心将开发数学方法，用于同类表示，分析 用于纯度评估和质量控制的单一同类溶液，并对制备的标准溶液进行测试 对照经认证的标准参考物质，包括印刷电路板校准混合物。具体目标2是 开发用于实验动物暴露研究的多氯联苯同系物混合物。分析的核心 我将开发数学方法来设计曝光混合物并测试准备好的混合物与 经认证的标准参考物质。具体目标3是环境和实验室的分析 一套多氯联苯同类产品的样品(&gt；120)。分析核心将提取和分析一个 同类者特定的基础。应用在目标1下制定的主要校准标准，样品将 用同位素稀释法和内标法定量。内标和替代物 根据目标1制定的回收标准将用于准确确定样品中的多氯联苯浓度。 将使用性能标准注射和 经认证的标准物质的分析。将对整个印刷电路板套件进行一些分析 同系物，包括暴露在目标2所述混合物中的实验动物，包括来自 人类和非实验室动物。其他样本将分析特定的同系物和代谢物 侧重于同类特定毒理学的调查人员的支持。特定目标4涉及提取， 未知代谢物的鉴定和定量。分析核心将决定纯度 合成的代谢物和相关的分析标准，然后分析羟基多氯联苯和其他多氯联苯 代谢物。应sbrp调查员的要求，分析核心还将培训人员并协助 抽样设计与实现。具体目标5是数据库系统的设计和维护。一个 将使用具有自动备份和密码保护的冗余存储架构，以允许调查人员 下载由分析核心制作的数据和报告。

The Analytical Core provides routine and non-routine analytical service to the University of Iowa research program. The core will provide expert staff, equipment, and instrumentation in line with five Aims. Specific Aim 1 is the development of analytical and quality control/assurance standards. In close collaboration with the Synthesis Core, which is responsible for acquiring and archiving individual compound standards and preparing stock solutions, the Analytical Core will develop the mathematical methods for congener representation, analyze single congener solutions for purity assessment and quality control, and test the prepared standard solutions against certified Standard Reference Materials, including PCB calibration mixtures. Specific Aim 2 is the development of a mixture of PCB congeners for laboratory animal exposure studies. The Analytical Core will develop the mathematical methods to design the exposure mixture and test the prepared mixture against certified Standard Reference Materials. Specific Aim 3 is the analysis of environmental and laboratory samples for a suite of PCB congeners (>120). The Analytical Core will extract and analyze PCBs on a congener-specific basis. Applying the primary calibration standard developed under Aim 1, samples will be quantified by the isotope dilution method and the internal standard method. Internal Standards and .Surrogate Recovery Standards developed under Aim 1 will be used to precisely determine PCB concentrations in samples. Methods for analysis on a congener-specific basis will be assessed using performance standard injections and analysis of certified Standard Reference Materials. Some analyses will be performed on the entire suite of PCB congeners, including laboratory animals exposed to the mixture described in Aim 2 and including blood from humans and non-laboratory animals. Other samples will be analyzed for specific congeners and metabolites in support of investigators focused on congener-specific toxicology. Specific Aim 4 involves the extraction, identification, and quantification of unknown metabolites. The Analytical Core will determine purity of synthesized metabolites and associated analytical standards, then analyze for hydroxylated PCBs and other PCB metabolites. As requested by the sbrp investigators, the Analytical Core will also train personnel and assist in sampling design and implementation. Specific Aim 5 is the design and maintenance of a database system. A redundant storage architecture with automatic backup and password protection will be used to allow investigators to download data and reports produced by the Analytical Core.