WNPRC ASSAY SERVICES UNIT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：为核心、CTSA临床转化研究、校园和非东道主研究人员提供复杂和专业的分析资源和专业知识。 进展和关切： 从2009年1月1日至2009年12月31日，Assay Services已收到191,949美元作为我们服务的退款账户收入，319美元来自灵长类中心内的基本赠款账户。在这段时间里，我们进行了46,342次测定。化验服务公司的新举措是：1)为其他实验室提供蛋白质激素的放射免疫分析，作为服务费；2)开发和验证测定包括纹状体组织中的多巴胺及其代谢物和5-羟色胺在内的生物胺的方法；3)开发测定绒猴脑垂体和下丘脑灌流液中多巴胺的方法；4)开发超灵敏催产素的测定方法；5)开发催产素的研究工作；6)将人体样本作为威斯康星大学临床翻译科学奖的核心实验室进行翻译研究；6)开发LC/MS测定维生素D(1，25维生素D2和3，25(OH)维生素D2和维生素D2和3，25(OH)维生素D2和维生素D37)猕猴血清Kispeptin检测方法的建立和验证。 资源访问权限分配： 化验服务拥有国际和国内的客户基础。在过去的一年里，我们为来自许多不同州和几个国家的调查人员测试了样本。我们主要为基于NIH的资助提供服务，使用非人类灵长类样本进行荷尔蒙测定，最近还提供翻译研究。我们在3月份提交了一份NCRR共享仪器拨款(S10)的提案，用于LC/MS/MS，以增强我们同时测量维生素D和其他多种类固醇的能力。 化验服务公司有一个便于科学界评估的网站： Http://ink.primate.wisc.edu/~assay/assay.php 传播： 我们有很多客户通过我们的网站与我们联系。此外，我们还参加美国灵长类动物学家协会、国际灵长类学会、行为神经内分泌学学会和内分泌学会等会议，让非人类灵长类动物群体了解我们的服务。我们也接受客户的推荐，由以前的客户。 培训： 化验服务继续培训研究生、技术支持人员、博士后和科学家以自助方式运行自己的样本。纽约石溪大学的一名博士后和芬兰赫尔辛基大学的一名研究生花了几个月的时间验证和分析了几种自由放养的狐猴的粪便样本。威斯康星大学人类学研究生对阿卢阿塔皮格拉的粪便样本进行了睾酮和皮质醇的检测。加州大学伯克利分校的一名研究生花了六个月的时间培训和分析红色根瘤猴的样本。心理学博士后，受训分析尿样中的催产素和加压素。威斯康星大学神经科学项目的一名研究生学习了高效液相色谱和电化学检测小鼠纹状体组织。另一名研究生为加利福尼亚老鼠培训并分析了皮质酮、睾酮和黄体酮的血清样本。此外，我们还培训了寺泽惠研究团队的两名研究生和一名博士后。在Assay Services内部，我们培训了一名在实验室工作的本科生和一名高中生进行暑期培训。化验服务公司已经开始培训加州灵长类中心的技术人员，开发通过高效液相色谱电化学检测来检测血浆儿茶酚胺的方法。 出版物： *Ziegler TE，Prudom SL，Zahed SR。常见绒猴雄性育儿行为和生理的变异。我是J·哈姆·比奥。2009年11月-12月；21(6)：739-44。PMID：19367571 *雅培公司，塔兰塔尔公司，杜梅西奇公司。产前雄激素诱导的雌性恒河猴多囊卵巢综合征的胎儿、婴儿、青少年和成年表型。我是J普利马托尔。2009年9月；71(9)：776-84.PMID：19367587 *Dumesic DA，Patankar MS，Barnett DK，Lesnick TG，Hutcher son BA，Abbott DH。孕期早期雄激素化导致成年雌性恒河猴卵巢储备减少。哼哼责备。2009年9月9日[印刷前电子出版]pmid：19740899 *Ziegler TE，Prudom SL，Zahed SR，Parlow AF，Wegner F.Prolactin在亲身经历过的绒猴(Callithrix Jacchus)中的男性育儿中的中介作用。霍姆·贝哈夫。2009年10月；56(4)：436-43。EPub 2009年8月5日PMID：19664636 范贝尔S，埃斯特拉达A，*齐格勒，斯特里尔KB。黑吼猴雄性生殖策略背后的社会和荷尔蒙机制。霍姆·贝哈夫。2009年10月；56(4)：355-63。EPub 2009年8月25.PMID：19712675 *Saltzman W，雅培卫生署循环皮质醇浓度升高对常见绒猴母性行为的影响。精神神经内分泌学。2009年4月10日[印刷前电子出版]pmid：19362777

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To provide core, CTSA clinical translational research, campus and non-host investigators with sophisticated and specialized assay resources and expertise. Progress and Concerns: From January 1, 2009 through December 31, 2009, Assay Services has received $191,949 as income from our charge back accounts for our service and $319 from charging base grant accounts within the Primate Center. We ran 46,342 determinations during this time. Assay Services' new initiatives have been 1) iodinating for other labs for their RIA of protein hormones as a fee for service, 2) development and validation of methods for determining biogenic amines including dopamine and its metabolites and serotonin in striatal tissue, 3) development of methods for measuring dopamine in marmoset pituitary and hypothalamic perfusates 4) developmental work on oxytocin for an ultrasensitive assay, 5) developmental work on oxytocin 6) running human samples as a core lab for the Clinical Translational Science Award at the University of Wisconsin for translational studies, 6) LC/MS development for measuring Vitamin D (1,25 Vitamin D2&3, 25(OH) Vitamin D2&3, 7) development and validation of a kisspeptin assay for rhesus serum. Allocation of Resource Access: Assay Services has an international and national client base. During the past year we ran samples for investigators from many different states and several countries. We primarily provide services for NIH based grants using nonhuman primate samples for hormonal determinations and more recently for translational studies. We submitted a proposal for the NCRR shared instrumentation grant (S10) in March for a LC/MS/MS to enhance our capability to measure the Vitamin Ds simultaneously and other multisteroids. Assay Services has a website that is easily assessable to the scientific community: http://ink.primate.wisc.edu/~assay/assay.php Dissemination: We have many clients who contact us via our website. Additionally, we attend meetings such as the American Society of Primatologists, International Primatological Society, Society of Behavioral Neuroendocrinology, and the Endocrine Society to make the nonhuman primate community aware of our services. We also receive clients from recommendations by previous clients. Training: Assay Services continues to train graduate students, technical support, post doc and scientists to run their own samples as self-service. A postdoctoral student from the Stony Brook University, New York, spent several months validating and analyzing fecal samples from several species of free-ranging lemurs along with a graduate student from the University of Helsinki, Finland. A University of Wisconsin anthropology graduate student trained and ran fecal samples for testosterone and cortisol from Alouatta pigra. A graduate student at UC-Berkeley spent six months training and analyzing samples from red colobus monkeys. A postdoc from Psychology trained in analysis of urine samples for oxytocin and vasopressin. A graduate student with the UW neuroscience program learned HPLC with electrochemical detection for mouse striatal tissue. Another graduate student trained and analyzed serum samples for corticosterone, testosterone and progesterone for Californica mus. Additionally, we have trained two graduate students and a postdoctoral student from Ei Terasawa's research team. Within Assay Services, we trained one undergraduate student for working in the lab and a high school student for summer time training. Assay Services has begun to train technical staff at the California Primate Center to develop plasma catecholamines detection by HPLC electrochemical detection. PUBLICATIONS: *Ziegler TE, Prudom SL, Zahed SR. Variations in male parenting behavior and physiology in the common marmoset. Am J Hum Biol. 2009 Nov-Dec;21(6):739-44. PMID: 19367571 *Abbott DH, Tarantal AF, Dumesic DA. Fetal, infant, adolescent and adult phenotypes of polycystic ovary syndrome in prenatally androgenized female rhesus monkeys. Am J Primatol. 2009 Sep;71(9):776-84.PMID: 19367587 *Dumesic DA, Patankar MS, Barnett DK, Lesnick TG, Hutcherson BA, Abbott DH. Early prenatal androgenization results in diminished ovarian reserve in adult female rhesus monkeys. Hum Reprod. 2009 Sep 9. [Epub ahead of print]PMID: 19740899 *Ziegler TE, Prudom SL, Zahed SR, Parlow AF, Wegner F. Prolactin's mediative role in male parenting in parentally experienced marmosets (Callithrix jacchus). Horm Behav. 2009 Oct;56(4):436-43. Epub 2009 Aug 5. PMID: 19664636 Van Belle S, Estrada A, *Ziegler TE, Strier KB. Social and hormonal mechanisms underlying male reproductive strategies in black howler monkeys (Alouatta pigra). Horm Behav. 2009 Oct;56(4):355-63. Epub 2009 Aug 25.PMID: 19712675 *Saltzman W, Abbott DH. Effects of elevated circulating cortisol concentrations on maternal behavior in common marmoset monkeys (Callithrix jacchus). Psychoneuroendocrinology. 2009 Apr 10. [Epub ahead of print ] PMID: 19362777