WNPRC ASSAY SERVICES UNIT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 目的：为核心、校园和非东道主研究者提供复杂和专业的分析资源和专业知识。 进展和关切 从2008年1月1日到2009年12月31日，分析服务已经收到了173,710美元的收入，从我们的收费帐户为我们的服务和5,878美元的收费基础赠款帐户内的灵长类动物中心。 在此期间，我们进行了43，881次测定。 Assay Services的新举措包括：1）为其他实验室提供蛋白质激素RIA的碘化，作为服务费; 2）开发和验证人类和其他灵长类动物尿中儿茶酚胺的方法; 3）开发用于测量药物的HPLC方法：奥美拉唑（人）和吡格列酮（恒河猴），4）用于超灵敏测定的催产素的开发工作，5）在威斯康星州大学作为临床转化科学奖的核心实验室运行人类样品进行转化研究，6）食蟹猴中肾上腺类固醇途径的LC/MS分析和7）恒河猴血清kisspeptin测定的开发和验证。 资源访问的分配 分析服务有一个国际和国家的客户群。 在过去的一年里，我们为来自许多不同州和几个国家的调查人员运行了样本。 我们主要为NIH的赠款提供服务，使用非人灵长类动物样本进行激素测定，最近用于转化研究。 分析服务有一个网站，科学界很容易访问： http://ink.primate.wisc.edu/~assay/assay.php 传播 我们有很多客户通过我们的网站联系我们。 此外，我们参加会议，如灵长类动物学家，国际灵长类动物学会，行为神经内分泌学会和内分泌学会，使非人类灵长类动物社区了解我们的服务。 我们也接受以前客户的推荐。 培训 分析服务继续培训研究生，技术支持，博士后和科学家运行自己的样品作为自助服务。 来自纽约斯托尼布鲁克大学的博士后Stacey Tecot花了几个月的时间验证和分析了几种自由放养的狐猴的粪便样本。 Sarie货车贝儿，威斯康星州大学  人类学，训练和运行粪便样本睾丸激素和皮质醇从阿劳阿塔pigra。Elizabeth Becker培训并分析了加利福尼亚州mus的皮质酮，睾酮和孕酮的血清样本。此外，我们还培训了两名研究生，Kathryn Guerriero，Brian Kenealy和Ei Terrasawa研究团队的本科生Nicholas Shiel。 在分析服务中，我们培训了两名本科生，Nancy Stevens和Dan Champeau基本实验室工作和HPLC。 Dan Champeau也做了研究学分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To provide core, campus and non-host investigators with sophisticated and specialized assay resources and expertise. Progress and Concerns From January 1, 2008 through December 31, 2009, Assay Services has received $173,710 as income from our charge back accounts for our service and $5,878 from charging base grant accounts within the Primate Center. We ran 43,881 determinations during this time. Assay Services' new initiatives have been 1) iodinating for other labs for their RIA of protein hormones as a fee for service, 2) development and validation of methods for urinary catecholamines for humans and other primates, 3) development of HPLC methods for measuring the drugs: omedprizole (humans) and pioglitazone (rhesus), 4) developmental work on oxytocin for an ultrasensitive assay, 5) running human samples as a core lab for the Clinical Translational Science Award at the University of Wisconsin for translational studies, 6) LC/MS analysis of adrenal steroid pathways in cycnomolgus macaques and 7) development and validation of a kisspeptin assay for rhesus serum. Allocation of Resource Access Assay Services has an international and national client base. During the past year we ran samples for investigators from many different states and several countries. We primarily provide services for NIH based grants using nonhuman primate samples for hormonal determinations and more recently for translational studies. Assay Services has a website that is easily assessable to the scientific community: http://ink.primate.wisc.edu/~assay/assay.php Dissemination We have many clients who contact us via our website. Additionally, we attend meetings such as the American Society of Primatologists, International Primatological Society, Society of Behavioral Neuroendocrinology, and the Endocrine Society to make the nonhuman primate community aware of our services. We also receive clients from recommendations by previous clients. Training Assay Services continues to train graduate students, technical support, post doc and scientists to run their own samples as self-service. Stacey Tecot, postdoctoral student from the Stony Brook University, New York, spent several months validating and analyzing fecal samples from several species of free-ranging lemurs. Sarie Van Belle, University of Wisconsin  Anthropology, trained and ran fecal samples for testosterone and cortisol from Alouatta pigra. Elizabeth Becker trained and analyzed serum samples for corticosterone, testosterone and progesterone for Californica mus. Additionally, we have trained two graduate students, Kathryn Guerriero, Brian Kenealy, and an undergraduate student, Nicholas Shiel from Ei Terrasawa's research team. Within Assay Services, we trained two undergraduate students, Nancy Stevens and Dan Champeau in basic lab work and HPLC. Dan Champeau also did research credits.