Genome wide profiling of histone variants in colorectal cancer cells

结直肠癌细胞组蛋白变异的全基因组分析

• 批准号: 8349395
• 负责人: YAMINI P DALAL
• 金额: $ 49.99万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8349395
• 关键词: Address; Cancer cell line; Chromatin Structure; Chromosomal Instability; Colorectal Cancer; Epigenetic Process; Failure; Functional RNA; Future; Genes; Genome; Goals; Histones; Lead; Location; Maps; Methods; Outcome; Process; Specificity; Tumor Biology; Variant; cancer cell; centromere protein A; genome-wide; new technology; tumor; tumorigenic

We have mapped a unique histone variant genome wide in colorectal cancer cell lines and found that it is clustered tightly at epigenetically distinctive regions. We have mapped these regions further using cytological methods and found a subset that enriched in regions implicated in tumor biology. Future goals involve mapping histone variants in tumors in order to track progression of aberrant chromatin structures as a consequence of the tumorigenic process, and discovering remodelers and processes involved in the mis-localization.

我们已经在结直肠癌细胞系中绘制了一个独特的组蛋白变体基因组，并发现它在表观遗传学上的独特区域紧密聚集。我们已经使用细胞学方法进一步绘制了这些区域，并发现了一个富含肿瘤生物学相关区域的子集。未来的目标包括绘制肿瘤中的组蛋白变体，以跟踪肿瘤发生过程导致的异常染色质结构的进展，并发现错误定位中涉及的重塑和过程。