Analysis of centromere identity and structure in human cells

人类细胞着丝粒的身份和结构分析

• 批准号: 10262264
• 负责人: YAMINI P DALAL
• 金额: $ 55.54万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10262264
• 关键词: Binding; Cell Cycle; Cell Cycle Regulation; Cells; Centromere; Chromosome Fragile Sites; Chromosomes; Complex; Computer Models; Data; Euchromatin; Foundations; Genetic Transcription; Genome; Goals; Histone H3; Human; Kinetochores; Maps; Mediating; Mitosis; Mitotic; Mutation; Normal Cell; Property; Proteins; Site; Structure; Variant; cancer cell; centromere protein A; daughter cell; in vivo; recruit

We have intrinsic properties of components of human centromeres, finding them to be surprisingly elastic. These elastic components become rigidified when incorporated into active kinetochores, and suppress centromeric accessibility, thereby suppressing transcription. These data have massive consequences for translocations in cancer cells where we have mapped centromere-like domains in euchromatin, which are sites of fragility. The current focus is to establish whether centromere-like domains in fragile sites can alter the structural stability of those sites.

我们有人类着丝粒组成部分的内在属性，发现它们具有惊人的弹性。当这些弹性成分被整合到活性动粒中时变得刚性化，并抑制着丝粒的可接近性，从而抑制转录。这些数据对癌细胞中的易位产生了巨大的影响，我们已经在常染色质中绘制了着丝粒样结构域，这是脆弱性的位点。目前的重点是确定是否在脆性网站的着丝粒样结构域可以改变这些网站的结构稳定性。