Genome wide profiling of histone variants in tumors

肿瘤中组蛋白变异的全基因组分析

• 批准号: 8938009
• 负责人: YAMINI P DALAL
• 金额: $ 36.81万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8938009
• 关键词: Address; Cancer cell line; Centromere; Chromatin Structure; Chromosomal Instability; Chromosome Fragile Sites; Colorectal Cancer; Epigenetic Process; Failure; Functional RNA; Future; Genes; Genome; Goals; Histones; Human; Lead; Location; Maps; Methods; Modification; Outcome; Postdoctoral Fellow; Process; Specificity; Variant; Work; cancer cell; centromere protein A; follow-up; genome-wide; new technology; response; tumor; tumor progression; tumorigenic

We have mapped unique histone variant genome wide in colorectal cancer cell lines and found that that they cluster at a subset of epigenetically distinctive regions. We have mapped these regions further using cytological methods and discovered a new centromere-like region in a fragile chromosome site encompassing the myc locus. Future goals involve mapping histone variants in tumors in order to track progression of aberrant chromatin structures as a consequence of the tumorigenic process, and discovering remodelers and processes involved in the mis-localization. We are also purifying large quantities of histone variants and examining their modification status in response to tumor progression.

我们已经在结直肠癌细胞系中绘制了独特的组蛋白变体基因组，并发现它们聚集在表观遗传学独特区域的子集上。我们已经绘制了这些地区进一步使用细胞学方法，并发现了一个新的着丝粒样的区域在一个脆弱的染色体站点包括myc基因座。未来的目标包括绘制肿瘤中的组蛋白变体，以跟踪肿瘤发生过程导致的异常染色质结构的进展，并发现错误定位中涉及的重塑和过程。我们还纯化了大量的组蛋白变体，并检查了它们对肿瘤进展的反应。