Analysis of centromere identity and structure in human cells
Analysis of centromere identity and structure in human cells
批准号:
7966218
负责人:
YAMINI P DALAL
金额:
$31.78万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
Atomic Force MicroscopyBindingCell CycleCell Cycle RegulationCellsCentromereCharacteristicsChromatinChromatin StructureChromosomesComplexEpigenetic ProcessFoundationsGenomeGoalsHela CellsHistone H3HistonesHumanKinetochoresLocationMass Spectrum AnalysisMeasurementMediatingMitosisMitoticPost-Translational Protein ProcessingProteinsRecruitment ActivityRestSpectrophotometryStructureVariantdaughter cell
中文摘要
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英文摘要
We have analyzed human centromeric chromatin containing CENPA from HeLa cells in culture using Chromatin IP followed by atomic force microscopy measurements and mass spectrophotometry. Thus far, we have identified that the CENPA chromatin structure is uniquely organized and appears to have fundamentally different characteristics from the rest of the chromatin which packages the genome. We have also purified CENPA histone from different points of the cell cycle that we plan to analyze by mass spectrometry to understand how epigenetic modifications of this protein may help restrict it to the centromere, and regulate it is only available at a particular point and location in the cell cycle
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