Analysis of centromere identity and structure in human cells

人类细胞着丝粒的身份和结构分析

• 批准号: 7966218
• 负责人: YAMINI P DALAL
• 金额: $ 31.78万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7966218
• 关键词: Atomic Force Microscopy; Binding; Cell Cycle; Cell Cycle Regulation; Cells; Centromere; Characteristics; Chromatin; Chromatin Structure; Chromosomes; Complex; Epigenetic Process; Foundations; Genome; Goals; Hela Cells; Histone H3; Histones; Human; Kinetochores; Location; Mass Spectrum Analysis; Measurement; Mediating; Mitosis; Mitotic; Post-Translational Protein Processing; Proteins; Recruitment Activity; Rest; Spectrophotometry; Structure; Variant; daughter cell

We have analyzed human centromeric chromatin containing CENPA from HeLa cells in culture using Chromatin IP followed by atomic force microscopy measurements and mass spectrophotometry. Thus far, we have identified that the CENPA chromatin structure is uniquely organized and appears to have fundamentally different characteristics from the rest of the chromatin which packages the genome. We have also purified CENPA histone from different points of the cell cycle that we plan to analyze by mass spectrometry to understand how epigenetic modifications of this protein may help restrict it to the centromere, and regulate it is only available at a particular point and location in the cell cycle

我们分析了来自HeLa细胞的含有CENPA的人着丝粒染色质， 使用染色质IP进行培养，随后进行原子力显微镜测量和质量分析。 分光光度法到目前为止，我们已经确定CENPA染色质结构是独特的， 有组织的，似乎有根本不同的特点，从其余的 包装基因组的染色质。我们还从不同的角度纯化了CENPA组蛋白 我们计划通过质谱分析来了解表观遗传学 这种蛋白质的修饰可能有助于将其限制在着丝粒上，并仅在着丝粒上调节它。 在细胞周期中的特定点和位置可用