IRON-SULFUR PROTEIN ASSEMBLY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 铁硫（[Fe-S]）簇是在各种生物中发现的简单无机基团。持有[Fe-S]簇的蛋白质可以具有各种独特的功能：电子传递，基因调控，环境传感和底物激活。特别是在几种[Fe-S]簇组装和转移蛋白系统中，IscU因其独特的生物学功能和灵活的特性而成为一种非常迷人的蛋白。到目前为止，人们认为ISCU组装[2Fe-2S]簇并将其转移到铁氧化还原蛋白。然而，涉及簇组装和转移的实际机制尚未在分子水平上描述。因此，通过对IscU的核磁共振（NMR）谱研究，我们期望能够确定IscU的结构动力学，从而解释IscU的分子机制。此外，我们还尝试分析全IscU的顺磁信号，并研究[Fe-S]团簇配位的特征模式。这可能阐明了IscU与[Fe-S]团簇之间相互作用的许多未知性质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Iron-sulfur ([Fe-S]) clusters are simple inorganic groups found in various organisms. The proteins holding the [Fe-S] clusters can have various unique functions: electron transfer, gene regulation, environmental sensing, and substrate activation. In particular, among several [Fe-S] cluster assembly and transfer protein systems, IscU is a very fascinating protein because of its unique biological functions and flexible characteristics. To date, it is believed that IscU assembles the [2Fe-2S] cluster and transfers it to ferredoxins. However, the actual mechanisms involved in the cluster assembly and transfer have not been described previously in the molecular level. Therefore, by investigating IscU with the nuclear magnetic resonance (NMR) spectroscopy, we are expecting that the structural dynamics of IscU can be determined, which may explain the molecular mechanisms of IscU as well. In addition, we are trying to analyze the paramagnetic signals of holo-IscU and to investigate the characteristic pattern of coordinating the [Fe-S] cluster. This may elucidate many unknown properties of the interaction between IscU and the [Fe-S] cluster.