TRACHEAL CARTILAGE RESHAPING

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 引言：气管前壁塌陷的常用手术修复技术（所谓的“开放技术”）通常与高发病率相关，长期支架植入可加重持续的气管炎症，并成为新狭窄的潜在来源。我们目前正在研究的激光剂量学和设计的内窥镜激光软骨整形手术装置，以尽量减少热损伤的软骨组织。目的：为了提供一个更好的理解激光诱导气管软骨重塑，我们研究了组织活力和激光剂量之间的关系，以及由于内窥镜激光照射的机械性能的变化。此外，我们设计了一种有效的内窥镜下激光气管软骨整形手术装置。材料和方法：将从新西兰兔中提取的气管软骨暴露于二极管激光（波长= 1450 nm，0.03-0.14W/mm 2，Canadian Corp. MA）3-5秒。使用ELF 3200机械测试仪器（EnduraTEC，明内通卡，MN）测量机械性能的变化，并使用激光扫描显微镜（LSM 510 Meta，Carl Zeiss Inc.，德国）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. INTRODUCTION:The usual surgical repair techniques (so-called "open technique") for anterior tracheal wall collapse are often associated with a high rate of morbidity, and prolonged stenting can aggravate the ongoing tracheal inflammation and serve as a potential source for a new stenosis. We are currently studying the laser dosimetry and the design of the surgical device for endoscopic laser cartilage reshaping minimizing the thermal damage of cartilage tissue. OBJECTIVE: In order to provide a better understanding of laser-induced tracheal cartilage reshaping, we examined the relationships between the tissue viability and the laser dosimetry as well as the change in mechanical property due to endoscopic laser exposure. In addition, we design an effective endoscopic surgical device for laser tracheal cartilage reshaping. MATERIALS AND METHODS: Tracheal cartilage extracted from New Zealand rabbits, were exposed to diode laser (wavelength =1450nm, 0.03-0.14W/mm2, Candela Corp. MA) for 3-5 seconds. The change in the mechanical property was measured using an ELF 3200 mechanical testing instrument (EnduraTEC, Minnetonka, MN) and its viability was determined using a laser scanning microscope (LSM510 Meta, Carl Zeiss Inc., Germany).