OPTICAL AND MOLECULAR APPROACHES TO THE STUDY OF CHEMICAL AGENTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的总体目标是应用现代光学和分子/细胞探针，通过使用2-氯乙基乙基硫醚（一种单功能硫芥（CEES））来研究SM对细胞结构和功能的影响。这项研究工作的目的是提高我们对SM暴露的主要细胞效应的理解。为此，SM暴露的细胞和分子事件的基本知识将作为未来生物传感器/探针开发的基石，并将促进更有效的治疗策略的开发。该项目的具体目标是：（1）在分子和细胞水平上检查CEES（半芥子气）的影响，特别是与细胞周期相关的细胞骨架，（2）确定Src在接触CEES后细胞骨架和粘附的解体中的潜在作用，（3）研究CEES在组织水平上与伤口愈合相关的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this project is to apply modern optical and molecular/cellular probes to study SM effects on cell structure and function by using 2-chloroethyl ethyl sulfide, a mono-functional sulfur mustard (CEES). This research effort is intended to enhance our understanding of the primary cellular effects of SM exposure. To this end, this fundamental knowledge of the cellular and molecular events underlying SM exposure will serve as a keystone to future biosensor/probe development and will facilitate the development of more effective treatment strategies. The specific objectives of this project are: (1) to examine the effects of CEES (half-mustard) at the molecular and cellular level, specifically, on the cytoskeleton in relationship to the cell cycle, (2) to determine a potential role for Src in the disorganization of the cytoskeleton and adhesion following exposure to CEES, (3) to study the effects of CEES at the tissue level as related to wound healing.