GENOME WIDE SIRNA SCREENING IDENTIFIES NOVEL REGULATORS OF MELANIN SECRETION

全基因组 SIRNA 筛选鉴定出黑色素分泌的新型调节因子

• 批准号: 8362629
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362629
• 关键词: Biotechnology; Cells; Confocal Microscopy; Funding; Genes; Goals; Grant; Image; Lasers; Melanins; Melanosomes; Membrane; Microscopy; Movement; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Screening procedure; Small Interfering RNA; Source; Time; United States National Institutes of Health; cost; genome-wide; melanocyte; novel; trafficking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A genome wide siRNA screen has identified several novel regulators of melanin secretion. The goal of this project is to determine the impact of selected genes on the trafficking of melanosomes within the cells. The goals of the project are to 1) image using standard microscopy the impact of select genes on melanosome trafficking; 2) image using confocal microscopy the impact of select genes on melanosome trafficking (localizaiton adjacent to the membrane); 3) image using confocal microscopy the real time movement of melanosomes within melanocytes.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 全基因组siRNA屏幕已经确定了黑色素分泌的几种新的调节因子。这个项目的目标是确定选定的基因对黑素小体在细胞内运输的影响。该项目的目标是1)使用标准显微镜成像特定基因对黑素小体运输的影响；2)使用共聚焦显微镜成像特定基因对黑素小体运输的影响(膜附近的定位)；3)使用共聚焦显微镜成像黑素细胞内黑素小体的实时运动。