X-RAY MICROSCOPY AND X-RAY MICRODIFFRACTION OF LANTHANUM DEPOSITS IN NEPHROTIC T

肾病 T 中镧沉积物的 X 射线显微镜和 X 射线微衍射

• 批准号: 8362306
• 负责人: SIMON J GEORGE
• 金额: $ 0.14万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362306
• 关键词: Animals; Autopsy; Chemicals; Deposition; End stage renal failure; Funding; Grant; Investigation; Lanthanum; Measures; Mesentery; National Center for Research Resources; Oral; Principal Investigator; Radiation; Reporting; Research; Research Infrastructure; Resources; Roentgen Rays; Sampling; Scanning Electron Microscopy; Source; Spectrum Analysis; United States National Institutes of Health; Visible Radiation; X ray microscopy; absorption; cost; inorganic phosphate; lanthanum carbonate; light microscopy; lymph nodes; pre-clinical; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Lanthanum carbonate (LaCO3) is an oral phosphate binder widely used in end-stage renal disease (ESRD). Preclinical animal studies reported the highest La concentrations outside the gut to be in mesenteric lymph nodes. We observed previously unreported La deposition visible by light microscopy and confirmed by scanning electron microscopy with energy dispersive x-ray spectroscopy in a mesenteric lymph node at autopsy following LaCO3 administration. Although LaCO3 is generally thought to be minimally absorbed, this demonstration suggests the need for further investigation of the extent and potential effects of such absorption. To this end samples will be measured using the microprobe to examine the extent of La distribution and the chemical forms of various deposits.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 碳酸镧（LaCO 3）是一种口服磷酸盐结合剂，广泛用于终末期肾病（ESRD）。临床前动物研究报告肠外的最高La浓度在肠系膜淋巴结中。我们观察到以前未报道的镧沉积可见光显微镜和证实的扫描电子显微镜能量色散X-射线光谱在肠系膜淋巴结尸检后，碳酸镧管理。虽然一般认为LaCO 3的吸收最低，但这一演示表明需要进一步研究这种吸收的程度和潜在影响。 为此，将使用微探针测量样品以检查La分布的程度和各种沉积物的化学形式。