REGULATION OF TRANSCRIPTS RELATED TO RAT ASN-LINKED GLYCAN BIOSYNTHESIS

与大鼠 ASN 连接聚糖生物合成相关的转录调控

• 批准号: 8363015
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363015
• 关键词: Anabolism; Animals; Castration; Data; Enzymes; Funding; Glycoproteins; Grant; Hormones; Hydrolase; Link; National Center for Research Resources; Pituitary Gland; Polysaccharides; Principal Investigator; Protein Isoforms; Proteins; Rattus; Regulation; Research; Research Infrastructure; Resources; Source; Technology; Tissues; Transcript; United States National Institutes of Health; cost; glycoprotein biosynthesis; glycosylation; glycosyltransferase; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for various glycan-related enzymes and proteins involved in Asn-linked glycoprotein biosynthesis have been quantitated by qRT-PCR from rat pituitary tissues derived from castrated and hormone treated animals. Parallel glycan structural data for secreted glycoprotein hormones derived from rat pituitary tissues (FSH isoforms) have been examined and characterized in the Campo lab. We have provided the qRT-PCR transcript data to Dr. Campo and they are comparing the alterations in FSH glycosylation with changes in expression of the glycosyltransferases and hydrolases involved in FSH glycosylation in response to castration and hormone treatment.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 已通过qRT-PCR从来自去势和激素处理动物的大鼠垂体组织中定量了参与Asn连接糖蛋白生物合成的各种聚糖相关酶和蛋白的转录水平。 在Campo实验室检查并表征了大鼠垂体组织（FSH亚型）分泌糖蛋白激素的平行聚糖结构数据。 我们已向Campo博士提供了qRT-PCR转录数据，他们正在比较FSH糖基化的变化与去势和激素治疗后FSH糖基化相关的糖基转移酶和水解酶表达的变化。