REGULATION OF TRANSCRIPTS RELATED TO RAT ASN-LINKED GLYCAN BIOSYNTHESIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在去势和激素处理的大鼠脑垂体组织中，参与ASN连接的糖蛋白生物合成的各种糖链相关酶和蛋白质的转录水平已经通过qRT-PCR进行了定量。来自大鼠脑垂体组织的分泌糖蛋白激素(FSH亚型)的平行糖蛋白结构数据已经在坎波实验室进行了检验和表征。我们已经向Campo博士提供了qRT-PCR转录本数据，他们正在比较FSH糖基化的变化与FSH糖基化所涉及的糖基转移酶和水解酶表达的变化，以响应去势和激素治疗。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for various glycan-related enzymes and proteins involved in Asn-linked glycoprotein biosynthesis have been quantitated by qRT-PCR from rat pituitary tissues derived from castrated and hormone treated animals. Parallel glycan structural data for secreted glycoprotein hormones derived from rat pituitary tissues (FSH isoforms) have been examined and characterized in the Campo lab. We have provided the qRT-PCR transcript data to Dr. Campo and they are comparing the alterations in FSH glycosylation with changes in expression of the glycosyltransferases and hydrolases involved in FSH glycosylation in response to castration and hormone treatment.