TRANSCRIPT ANALYSIS OF PROTEOGLYCAN BIOSYNTHETIC GENES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 参与小鼠蛋白多糖生物合成、核心蛋白和GAG识别的各种酶和蛋白质的转录水平正在通过qRT-PCR进行定量。罗伯特·林哈特(RPI)博士开发了蛋白多糖分析策略，获得了小鼠ES细胞和分化细胞衍生品中蛋白多糖组成的平行数据。我们正在协调从林哈特博士的团队收到的信息，并将结果与我们的qRT-PCR分析中的同等数据进行比较。转录水平正在与来自相同细胞群体的蛋白多糖相关的多糖结构进行比较，所有信息都将成为生物信息学模块的一部分，用于展示和进一步的相关性分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for various enzymes and proteins involved in murine proteoglycan biosynthesis, core proteins, and GAG recognition are being quantitated by qRT-PCR. Parallel data for proteoglycan composition in mouse ES cells and differentiated cell derivatives was obtained by Dr. Robert Linhardt (RPI), who has developed analytical strategies for proteoglycan analysis. We are coordinating the information received from Dr. Linhardt's group and comparing the results with equivalent data from our qRT-PCR analyses. Transcript levels are being compared with the glycan structures associated with proteoglycans from the equivalent cell populations and all of the information will become part of the bioinformatics module for display and further correlation analysis.