ANALYSIS OF TRANSCRIPTS INVOLVED IN GLYCOCONJUGATE SYNTHESIS IN D MELANOGASTER

黑腹果蝇糖复合物合成中涉及的转录本分析

• 批准号: 8363041
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363041
• 关键词: Caenorhabditis elegans; Cells; Collaborations; DNA Primers; Funding; Genes; Genomics; Glycoconjugates; Grant; National Center for Research Resources; Polysaccharides; Principal Investigator; RNA; Research; Research Infrastructure; Resources; Sampling; Source; Technology; Tissues; Transcript; United States National Institutes of Health; Validation; Zebrafish; cost; design; fly

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This collaboration involves transcript analysis of glycan-related genes in Drosophilia melanogaster. We have previously preformed qRT-PCR analysis on RNA isolated from cells or tissues from several mammalian samples, C. elegans and zebrafish, but this project with Drosophilia extends the platform even further. Dr. Tiemeyer and Dr. Katoh provided an initial list of D. melanogaster genes for primer design and fly genomic DNA for primer design and validation.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 这项合作涉及果蝇聚糖相关基因的转录分析。 我们之前已经对从几种哺乳动物样品的细胞或组织中分离的RNA进行了qRT-PCR分析，C. elegans和斑马鱼，但这个与果蝇的项目将平台扩展得更远。 Tiemeyer博士和Katoh博士提供了一份D.用于引物设计的黑腹果蝇基因和用于引物设计和验证的果蝇基因组DNA。