MICROARRAY VALIDATION OF DATA FROM WILD-TYPE AND MGAT5 KNOCK-OUT MOUSE TISSUES

野生型和 MGAT5 敲除小鼠组织数据的微阵列验证

• 批准号: 8363111
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363111
• 关键词: Cadherins; Cell Adhesion; Data; Funding; Genes; Grant; Knockout Mice; Microarray Analysis; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Source; Technology; Tissues; Transcript; United States National Institutes of Health; Validation; cost; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Genes that were determined to be increased or decreased in transcript level by microarray analysis of wild type and Mgat5 knock-out mouse tissues are being analyzed by qRT-PCR as a validation tool. Transcript levels are also being determined for a group of protocadherin genes from wild type and Mgat5 knock-out mouse tissues. Protocadherins comprise a subfamily within the larger cadherin superfamily and are thought to be involved in cell adhesion.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 通过对野生型和MGAT5敲除小鼠组织的微阵列分析确定的基因在转录水平上增加或降低，正在通过QRT-PCR作为验证工具分析。 还确定了来自野生型和MGAT5敲除小鼠组织的一组原钙粘着蛋白基因的转录水平。 原始蛋白包含较大的钙粘蛋白超家族中的亚家族，被认为参与细胞粘附。