REGULATION OF N-GLYCOSYLATION BY STIMULATION OF THE UNFOLDED PROTEIN RESPONSE

通过刺激未折叠蛋白反应来调节 N-糖基化

• 批准号: 8363016
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363016
• 关键词: Cultured Cells; Enzymes; Funding; Grant; Human; Laboratories; National Center for Research Resources; Nucleotide Biosynthesis; Pathway interactions; Polysaccharides; Principal Investigator; Process; Proteins; Regulation; Research; Research Infrastructure; Resources; Source; Structure; Technology; Transcript; United States National Institutes of Health; cost; glycosylation; lipooligosaccharide; response; sugar nucleotide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for various enzymes and proteins involved in interconversion of sugar nucleotides, biosynthesis of N-glycan lipid-linked oligosaccharides, and proteins involved in the unfolded protein response are being quantitated by qRT-PCR. The focus of the Lehrman laboratory is on unfolded protein response and changes in N-glycan structures as a result of perturbation of the lipid-linked oligosaccharide processing pathway. Human cultured cells will be treated with various agents to induce the unfolded protein response and transcript changes will be correlated with changes in LLO glycan pools.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 参与糖核苷酸相互转化的各种酶和蛋白质、N-葡聚糖脂联寡糖的生物合成以及参与未折叠蛋白反应的蛋白质的转录水平正在通过qRT-PCR进行定量。Lehrman实验室的重点是未折叠的蛋白质反应和N-糖链结构的变化，这是脂联低聚糖加工途径受到干扰的结果。人类培养的细胞将被不同的试剂处理以诱导未折叠的蛋白质反应，转录的变化将与LLO糖聚糖库的变化相关。