REGULATION OF N-GLYCOSYLATION BY STIMULATION OF THE UNFOLDED PROTEIN RESPONSE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 通过qRT-PCR定量参与糖核苷酸相互转化、N-聚糖脂质连接寡糖生物合成的各种酶和蛋白质以及参与未折叠蛋白质反应的蛋白质的转录水平。 Lehrman实验室的重点是未折叠蛋白质反应和N-聚糖结构的变化，这是脂质连接寡糖加工途径扰动的结果。 将用各种试剂处理人培养细胞以诱导未折叠蛋白应答，并且转录物变化将与LLO聚糖池的变化相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for various enzymes and proteins involved in interconversion of sugar nucleotides, biosynthesis of N-glycan lipid-linked oligosaccharides, and proteins involved in the unfolded protein response are being quantitated by qRT-PCR. The focus of the Lehrman laboratory is on unfolded protein response and changes in N-glycan structures as a result of perturbation of the lipid-linked oligosaccharide processing pathway. Human cultured cells will be treated with various agents to induce the unfolded protein response and transcript changes will be correlated with changes in LLO glycan pools.