QRT-PCR TRANSCRIPT ANALYSIS

QRT-PCR 转录本分析

• 批准号: 8363006
• 负责人: KELLEY W. MOREMEN
• 金额: $ 15.47万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363006
• 关键词: Cells; DNA biosynthesis; Detection; Enzymes; Funding; Genes; Grant; Measurement; Messenger RNA; Methods; Mus; National Center for Research Resources; Polysaccharides; Population; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Reverse Transcriptase Polymerase Chain Reaction; Source; Technology; Time; Transcript; United States National Institutes of Health; cost; design; embryonic stem cell; glycosylation; human embryonic stem cell; stem cell population

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for various enzymes and proteins involved in murine cellular glycosylation and recognition are being quantitated by real-time quantitative RT-PCR (qRT-PCR) by a method that allows for medium-throughput (~800 genes) transcript analysis. A unified strategy for primer design is being combined with optimized strategies for mRNA isolation, DNA synthesis, and qRT-PCR that will allow for high efficiency detection and measurement of transcripts for glycan-related genes over a range of seven orders of magnitude in abundance. The approach is being applied for the measurement of "glycan-related" transcripts from mouse and human embryonic stem (ES) cell populations and differentiated cell populations derived from mouse ES cells.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 通过实时定量RT-PCR（QRT-PCR）对参与鼠细胞糖基化和识别的各种酶和蛋白质的转录水平通过允许中等通知（〜800基因）转录本分析的方法进行定量。引物设计的统一策略与优化的mRNA隔离策略，DNA合成和QRT-PCR相结合，该策略将允许在七个巨大的范围内高效率检测和测量与聚糖相关基因的转录本。该方法正在用于测量来自小鼠和人类胚茎（ES）细胞群的“聚糖相关”转录物，以及源自小鼠ES细胞的分化细胞群体。