Core--Multiphoton imaging /electrophysiology

核心--多光子成像/电生理学

• 批准号: 7643770
• 负责人: ALEX L KOLODKIN
• 金额: $ 13.8万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7643770
• 关键词: Cells; Communities; Core Facility; Dyes; Electrophysiology (science); Equipment; Funding; Image; Incubators; Ions; Lasers; Life; Microscope; Monitor; Neurobiology; Other Resources; Photons; Physiologic pulse; Process; Protein Dynamics; Proteins; Pulse taking; Research Personnel; Resolution; Staging; Time; Tissues; Whole-Cell Recordings; fluorophore; instrumentation; intracellular protein transport; novel strategies; protein localization location; protein protein interaction; two-photon

The aim of the Multiphoton Imaging/EIectrophysiology Core is to provide instrumentation for analyzing protein localization, protein dynamics, and protein-protein interactions with high resolution. This facility will also allow users to perform time-lapse imaging of multiple fluorophores in living cells and tissues, and combine high resolution imaging of fluorescently tagged proteins or ion indicator dyes with electrophysiological monitoring of electrical activity. This Core is anchored by a Zeiss LSM 510 META NLO confocal microscope equipped with two visible lasers for performing traditional single-photon confocal imaging, and one near-infrared pulsed laser for two-photon imaging. In addition, the stage of this microscope has the capacity to either be enclosed to create an incubator for long-term time-lapse imaging, or to be surrounded by electrophysiology equipment to allow simultaneous whole-cell recording and imaging. The unique capabilities of this facility will serve as a resource for other NINDS funded investigators at JHU SOM and will establish new approaches for studying neurobiological processes that will benefit the greater JHU community. The equipment and capabilities of this core are not duplicated in any core facilities at JHU.

多光子成像/电生理学核心的目标是提供高分辨率的分析蛋白质定位、蛋白质动力学和蛋白质-蛋白质相互作用的仪器。该设施还将允许用户对活细胞和组织中的多个荧光团进行延时成像，并将荧光标记蛋白质或离子指示剂染料的高分辨率成像与电生理监测电活动相结合。该核心由蔡司LSM 510 Meta NLO共焦显微镜支撑，该显微镜配备了两个用于执行传统单光子共焦成像的可见光激光器，以及一个用于双光子成像的近红外脉冲激光器。此外，这种显微镜的工作台可以被封闭以创建长期延时成像的孵化器，或者被电生理设备包围以允许同时进行全细胞记录和成像。该设施的独特能力将为JHU SOM的NINDS资助的其他研究人员提供资源，并将为研究神经生物学过程建立新的方法，这将使JHU社区受益。这一核心的设备和能力不会在JHU的任何核心设施中重复。