Core--Multiphoton imaging /electrophysiology

多光子成像/电生理学核心的目的是提供用于分析蛋白质定位、蛋白质动力学和蛋白质-蛋白质相互作用的高分辨率仪器。该设施还将允许用户对活细胞和组织中的多个荧光团进行延时成像，并将荧光标记蛋白质或离子指示剂染料的高分辨率成像与电活动的电生理监测相结合。该核心由蔡司LSM 510 Meta NLO共焦显微镜固定，该显微镜配备两个用于执行传统单光子共焦成像的可见激光器和一个用于双光子成像的近红外脉冲激光器。此外，该显微镜的载物台可以封闭以创建用于长期延时成像的培养箱，或者被电生理设备包围以允许同时进行全细胞记录和成像。该设施的独特能力将作为JHU SOM其他NINDS资助的研究人员的资源，并将建立新的方法来研究神经生物学过程，这将使更大的JHU社区受益。该核心的设备和能力在JHU的任何核心设施中都没有重复。

The aim of the Multiphoton Imaging/EIectrophysiology Core is to provide instrumentation for analyzing protein localization, protein dynamics, and protein-protein interactions with high resolution. This facility will also allow users to perform time-lapse imaging of multiple fluorophores in living cells and tissues, and combine high resolution imaging of fluorescently tagged proteins or ion indicator dyes with electrophysiological monitoring of electrical activity. This Core is anchored by a Zeiss LSM 510 META NLO confocal microscope equipped with two visible lasers for performing traditional single-photon confocal imaging, and one near-infrared pulsed laser for two-photon imaging. In addition, the stage of this microscope has the capacity to either be enclosed to create an incubator for long-term time-lapse imaging, or to be surrounded by electrophysiology equipment to allow simultaneous whole-cell recording and imaging. The unique capabilities of this facility will serve as a resource for other NINDS funded investigators at JHU SOM and will establish new approaches for studying neurobiological processes that will benefit the greater JHU community. The equipment and capabilities of this core are not duplicated in any core facilities at JHU.