ORIENTATION OF FMO PROTEIN IN PHOTOSYNTHETIC BACTERIA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在光合生物中观察到的高激发能量转移效率依赖于单个蛋白质复合物中的最佳色素-蛋白质结合几何形状，也依赖于光系统的整体结构。在绿色硫细菌中，膜附着的Fenna-Matthews-Olson（FMO）天线蛋白作为“导线”将大的外周叶绿体天线复合物与嵌入细胞质膜中的反应中心（RC）连接起来。由叶绿体收集的能量通过FMO汇集到RC。 人们已经做出了巨大的努力来理解单个孤立粒子的结构和功能之间的关系。然而，FMO蛋白如何在体内与膜和叶绿体相互作用以维持特定的结构以确保高效的能量传递途径的问题尚未得到回答。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The high excitation energy transfer efficiency observed in photosynthetic organisms relies on the optimal pigment-protein binding geometry in the individual protein complexes and also on the overall architecture of photosystems. In green sulfur bacteria, the membrane-attached Fenna-Matthews-Olson (FMO) antenna protein functions as a "wire" to connect the large peripheral chlorosome antenna complex with the reaction center (RC), which is embedded in the cytoplasmic membrane. Energy collected by the chlorosome is funneled through the FMO to the RC. Significant effort has been expanded to understand the relationship between structure and function of the individual isolated particles. The question of how the FMO protein interacts with the membrane and the chlorosome in vivo to maintain a specific architecture to ensure the highly efficient energy transfer pathway, however, has not been answered.