Molecular Transducers of Physical Activity Clinical Centers (U01) - Cedars -Sinai Medical Center

身体活动分子传感器临床中心 (U01) - Cedars -Sinai Medical Center

• 批准号: 10782038
• 负责人: Nicolas Musi
• 金额: $ 39.6万
• 依托单位: CEDARS-SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-06 至 2023-09-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10782038
• 关键词: Molecular; Transducers; Physical; Activity; Clinical

Project Summary/Abstract: This application is for the formation of an Adult Clinical Center in response to RFA-RM-15-015 “Molecular Transducers of Physical Activity Clinical Centers (U01)” (MoTrPAC). Physical activity is an important lifestyle behavior that has been shown to be associated with morbidity and mortality from numerous chronic diseases. This has led to current public health recommendations of 150 min/wk of moderate-intensity physical activity or 75 min/wk of vigorous physical activity to achieve significant health- related benefits, and for recommendations to engage in resistance exercise. Despite the abundance of data to support physical activity as a key lifestyle factor that can prevent, delay, or reverse the onset of a variety of health-related conditions, the specific underlying physiological mechanisms and pathways by which this occurs are not fully established. Our team of investigators is well-positioned to be an Adult Clinical Center and achieve the goals and objectives outlined in this RFA. We have the capacity in Pittsburgh to recruit, retain, train, and assess the 450 participants that are required at each Adult Clinical Center. We will recruit 450 participants at the Pittsburgh Adult Clinical Center, which will include untrained subjects who will be randomized to the intervention (72% of the sample, N=324) or control condition (12% of the sample, N=54) and trained subjects (16% of the sample, N=72). Untrained participants will be randomized to one of the following for a period of 12 weeks: 1) Control (UN- CON) that will maintain their current level of physical activity; 2) Moderate-Intensity Physical Activity (UN-MOD) that will progress to 150 min/wk of supervised moderate-intensity endurance physical activity; 3) Vigorous- Intensity Physical Activity (UN-VIG) that will progress to 75 min/wk of supervised vigorous-intensity endurance physical activity; 4) Resistance Training (UN-RES) that will engage in supervised resistance exercise. In addition, exercise trained participants (50% endurance trained, 50% resistance trained) will be recruited and will undergo baseline testing only. Thus, we propose to form an Adult Clinical Center at the University of Pittsburgh to achieve the following objectives of MoTrPAC: 1. To recruit, retain, and collect data on 450 participants (378 untrained and 72 trained) at the Pittsburgh site that will contribute to the 2,700 total participants across the Adult Clinical Centers. 2. To demonstrate the effectiveness of our training program, we will compare changes in cardiorespiratory fitness in untrained participants randomized to 12-weeks of moderate-intensity endurance exercise training (UN-MOD), vigorous-intensity endurance exercise training (UN-VIG), resistance training (UN-RES) or control (UN-CON). 3. To demonstrate the effectiveness of our training program, we will compare changes in musculoskeletal fitness in untrained participants randomized to 12-weeks of UN-MOD, UN-VIG, UN-RES, or UN-CON. 4. To compare cardiorespiratory fitness in participants recruited as endurance trained to participants randomized to UN-MOD and UN-VIG following 12 weeks of training. 5. To compare musculoskeletal fitness in participants recruited as endurance trained to participants randomized to UN-RES following 12 weeks of training. 6. To collect phenotypic data that will allow for characterization of the study participants. These data will be provided to the Coordinating Center and utilized by the Bioinformatics Center, Metabolomics and Proteomics Analysis Sites, and Genomics, Epigenomics, and Transcriptomics Analysis Sites. 7. To collect biological data according to the study protocol that will provide data to examine the molecular and cellular transducers in response to acute and chronic exposure to endurance or resistance exercise training. These data will be provided to the Coordinating Center and utilized by the Bioinformatics Center, Metabolomics and Proteomics Analysis Sites, and Genomics, Epigenomics, and Transcriptomics Sites.

项目概要/摘要： 本申请旨在成立成人临床中心，以响应RFA-RM-15-015“分子 身体活动临床中心传感器（U 01）”（MoTrPAC）。 体力活动是一种重要的生活方式行为，已被证明与发病率相关， 许多慢性疾病的死亡率。这导致目前的公共卫生建议为150分钟/周 中等强度的体力活动或75分钟/周的剧烈体力活动，以实现显着的健康- 相关的好处，并建议从事阻力运动。尽管有大量的数据， 支持体育活动作为一个关键的生活方式因素，可以预防，延迟或逆转各种疾病的发生 与健康相关的疾病，发生这种情况的具体潜在生理机制和途径 还没有完全建立。 我们的研究团队有能力成为成人临床中心，并实现目标， 本RFA中列出的目标。我们在匹兹堡有能力招募、留住、培训和评估这450人 每个成人临床中心需要的参与者。我们将招募450名参与者在匹兹堡成人 临床中心，将包括将被随机分配到干预组的未经培训的受试者（72%的 样品的12%，N=324）或对照条件（样品的12%，N=54）和训练的受试者（样品的16%，N=72）。 未经训练的参与者将被随机分配至以下之一，持续12周： CON）将保持他们目前的体力活动水平; 2）中等强度体力活动（UN-MOD） 这将进展到150分钟/周的监督中等强度的耐力体力活动; 3）充满活力- 强度体力活动（UN-VIG），将进展到75分钟/周的监督剧烈强度耐力 身体活动; 4）阻力训练（UN-RES），将参与监督阻力练习。在 此外，还将招募经过运动训练的参与者（50%耐力训练，50%阻力训练）， 仅进行基线测试。 因此，我们建议在匹兹堡大学成立一个成人临床中心，以实现以下目标 MoTrPAC的目标： 1.在匹兹堡研究中心招募、保留和收集450名参与者（378名未经培训，72名经过培训）的数据 这将有助于整个成人临床中心的2,700名参与者。 2.为了证明我们的训练计划的有效性，我们将比较心肺功能的变化， 随机接受12周中等强度耐力运动训练的未接受训练的参与者的健康状况 （UN-MOD）、高强度耐力运动训练（UN-VIG）、阻力训练（UN-RES）或对照 （UN-CON）。 3.为了证明我们的训练计划的有效性，我们将比较肌肉骨骼的变化， 在未经训练的参与者中，随机分配到12周的UN-MOD，UN-VIG，UN-RES或UN-CON。 4.比较招募的接受耐力训练的参与者与参与者的心肺适能 在12周的培训后随机分配到UN-MOD和UN-VIG。 5.比较招募的接受耐力训练的参与者与随机分配的参与者的肌肉骨骼健康 经过12周的培训后， 6.收集表型数据，以便对研究受试者进行表征。这些数据将 提供给协调中心，并由生物信息学中心、代谢组学和蛋白质组学使用 分析位点和基因组学、表观基因组学和转录组学分析位点。 7.根据研究方案收集生物学数据，为检查分子和 细胞传感器对急性和慢性耐力或阻力运动训练的反应。 这些数据将提供给协调中心，并由生物信息学中心使用， 代谢组学和蛋白质组学分析网站，以及基因组学、表观基因组学和转录组学网站。