Supplement to Center for Excellence in Diabetes and Obesity Research: Implementing Biomechanics Instrumentation in the Diabetes and Obesity Center

摘要 COBRE奖（P30 GM 127607）主要关注心血管疾病的代谢机制。 该中心目前的目标是支持分子，细胞和翻译研究， 代谢紊乱的心血管原因和后果-特别是与 糖尿病和肥胖症。这一专题重点有助于分享资源和专门知识，并促进 多学科研究项目的发展，有助于长期稳定的 中心为了支持其研究人员，特别是我们的ESI成员的研究活动，该中心已 创建并维持（近15年）关键核心设施，其中包括流式细胞术，成像和 病理学、手术模型、心血管功能和免疫代谢聚焦质谱。因此，我们认为， 该中心具有独特的定位，以建立新的模式，在心脏代谢健康，同时培育 ESI/NI教师的发展。心力衰竭和心室重构是中心最常用的模型。 这些模型涉及心肌的显著生物力学变化;然而， 系统上的健康科学中心校园询问这种变化的生物力学。请求的 文书是相关的，并分配给这个父COBRE奖。本补充申请概述了中心的 需要布鲁克JPK NanoWizard 4 XP AFM（原子力显微镜）。这一最先进的系统具有 z轴噪声分辨率为30 pm，xy轴噪声分辨率为90 pm，同步荧光/透射光 具有图像叠加功能的显微镜、高速信号捕获、用户可编程软件（成像的理想选择 该中心拥有多个用户（多个设备），并与中心现有的尼康A1共焦显微镜兼容。这些 这些功能和许多其他功能将很好地服务于COBRE-funded Center的用户，但也适用于其他中心， 目前还没有原子力显微镜系统存在的校园部门。所请求的系统 将解决中心和校园其他地方的调查人员的重大未满足的需求。中心 将利用现有的基础设施，并在专用空间安装新系统。该系统将被管理 经验丰富的团队。将提供强有力的机构支持，以确保有效和高效的运作 这个系统在中心。考虑到几乎不存在的运营成本， 由于系统升级和相对简单，该仪器在安装后将自我维持。 因此，布鲁克JPK NanoWizard 4 XP AFM将对科学产生直接和持续的影响 在中心内和整个健康科学中心校园的其他实验室进行。这 该系统也将提供给其他合格的用户，特别是那些在更大的由IDEA资助的KY-INBRE内的用户 在肯塔基州的联邦网络。

Abstract The parent COBRE award (P30 GM127607) focuses on the metabolic mechanisms of cardiovascular disease. The current objectives of the Center are to support molecular, cellular, and translational investigations into the cardiovascular causes and consequences of derangements in metabolism—notably those associated with diabetes and obesity. This thematic focus facilitates the sharing of both resources and expertise and promotes the development of multi-disciplinary research projects that have contributed to the long-term stability of the Center. To support the research activities of its investigators, particularly our ESI members, the Center has created and sustained (for nearly 15 years) key core facilities, which include flow cytometry, imaging and pathology, surgical models, cardiovascular function, and immunometabolic-focused mass spectrometry. Hence, the Center is uniquely positioned to establish new paradigms in cardiometabolic health while nurturing the development of ESI/NI faculty. Heart failure and ventricular remodeling are the most used models in the Center. These models involve significant biomechanical changes to the myocardium; however, there is no suitable system on the Health Sciences Center Campus to interrogate such changes in biomechanics. The requested instrument is relevant and allocable to this parent COBRE award. This supplement request outlines the Center’s need for a Bruker JPK NanoWizard 4 XP AFM (atomic force microscope). This state-of-the-art system features 30 pm noise resolution in the z-axis and 90 pm in the xy-axes, simultaneous fluorescence/diascopic light microscopy with image overlay, high speed signal capture, user programmable software (ideal for imaging facilities with multiple users), and compatibility with the Center’s existing Nikon A1 confocal microscope. These features, and many others, will serve well the users in the COBRE-funded Center, but also in other Centers and Departments across campus—where currently no atomic force microscopy system exists. The requested system will address significant unmet needs of investigators within the Center and elsewhere on campus. The Center will leverage existing infrastructure and install the new system in dedicated space. The system will be managed by an experienced team. Robust institutional support will be provided to assure effective and efficient operation of this system in the Center. Given the nearly nonexistent costs of operation, the lifetime support for software upgrades, and the relative simplicity of the system, this instrument will be self-sustainable after installation. Hence, the Bruker JPK NanoWizard 4 XP AFM will create both immediate and sustained impacts on the science performed both within the Center and in other laboratories across the Health Sciences Center Campus. This system will also be available for other qualified users, particularly those within the larger IDeA-funded KY-INBRE network in the Commonwealth of Kentucky.