Supplement to Center for Excellence in Diabetes and Obesity Research: Implementing Biomechanics Instrumentation in the Diabetes and Obesity Center

糖尿病和肥胖研究卓越中心的补充：在糖尿病和肥胖中心实施生物力学仪器

• 批准号: 10582129
• 负责人: Steven P Jones
• 金额: $ 25万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10582129
• 关键词: 10 year old; Address; Administrative Supplement; Area; Atomic Force Microscopy; Award; Biocompatible Materials; Biomechanics; Biomedical Research; Cardiovascular Diseases; Cardiovascular Physiology; Cardiovascular system; Cells; Centers of Research Excellence; Cicatrix; Computer software; Core Facility; Data; Deposition; Development; Diabetes Mellitus; Elasticity; Environment; Faculty; Fees; Fibroblasts; Flow Cytometry; Fluorescence; Fresh Tissue; Funding; Future; Goals; Grant; Growth; Health; Health Sciences; Heart; Heart failure; Image; Individual; Infrastructure; Interdisciplinary Study; Investigation; Kentucky; Laboratories; Mass Spectrum Analysis; Metabolic; Metabolism; Microscope; Modeling; Modernization; Molecular; Myocardium; Noise; Obesity; Parents; Pathology; Positioning Attribute; Process; Research; Research Activity; Research Personnel; Research Project Grants; Research Support; Resolution; Resources; Scanning Probe Microscopes; Science; Services; Signal Transduction; Speed; Surgical Models; Surveys; System; Technology; Training; United States National Institutes of Health; Vendor; Ventricular Remodeling; base; cardiometabolism; cardiovascular health; cost; experience; flexibility; imaging facilities; instrument; instrumentation; interest; light microscopy; materials science; member; operation; programs; ranpirnase; success; sustainable resource; time use; user-friendly; wound healing

Abstract The parent COBRE award (P30 GM127607) focuses on the metabolic mechanisms of cardiovascular disease. The current objectives of the Center are to support molecular, cellular, and translational investigations into the cardiovascular causes and consequences of derangements in metabolism—notably those associated with diabetes and obesity. This thematic focus facilitates the sharing of both resources and expertise and promotes the development of multi-disciplinary research projects that have contributed to the long-term stability of the Center. To support the research activities of its investigators, particularly our ESI members, the Center has created and sustained (for nearly 15 years) key core facilities, which include flow cytometry, imaging and pathology, surgical models, cardiovascular function, and immunometabolic-focused mass spectrometry. Hence, the Center is uniquely positioned to establish new paradigms in cardiometabolic health while nurturing the development of ESI/NI faculty. Heart failure and ventricular remodeling are the most used models in the Center. These models involve significant biomechanical changes to the myocardium; however, there is no suitable system on the Health Sciences Center Campus to interrogate such changes in biomechanics. The requested instrument is relevant and allocable to this parent COBRE award. This supplement request outlines the Center’s need for a Bruker JPK NanoWizard 4 XP AFM (atomic force microscope). This state-of-the-art system features 30 pm noise resolution in the z-axis and 90 pm in the xy-axes, simultaneous fluorescence/diascopic light microscopy with image overlay, high speed signal capture, user programmable software (ideal for imaging facilities with multiple users), and compatibility with the Center’s existing Nikon A1 confocal microscope. These features, and many others, will serve well the users in the COBRE-funded Center, but also in other Centers and Departments across campus—where currently no atomic force microscopy system exists. The requested system will address significant unmet needs of investigators within the Center and elsewhere on campus. The Center will leverage existing infrastructure and install the new system in dedicated space. The system will be managed by an experienced team. Robust institutional support will be provided to assure effective and efficient operation of this system in the Center. Given the nearly nonexistent costs of operation, the lifetime support for software upgrades, and the relative simplicity of the system, this instrument will be self-sustainable after installation. Hence, the Bruker JPK NanoWizard 4 XP AFM will create both immediate and sustained impacts on the science performed both within the Center and in other laboratories across the Health Sciences Center Campus. This system will also be available for other qualified users, particularly those within the larger IDeA-funded KY-INBRE network in the Commonwealth of Kentucky.

摘要

项目成果

Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction.

2 型糖尿病中的循环血管生成干细胞与血糖控制和内皮功能障碍有关。

• DOI: 10.1371/journal.pone.0205851
• 发表时间: 2018
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zafar,Nagma;Krishnasamy,SathyaS;Shah,Jasmit;Rai,SheshN;Riggs,DanielW;Bhatnagar,Aruni;O'Toole,TimothyE
• 通讯作者: O'Toole,TimothyE

Exercise Promotes Resolution of Acute Inflammation by Catecholamine-Mediated Stimulation of Resolvin D1 Biosynthesis.

运动通过儿茶酚胺介导的 Resolvin D1 生物合成刺激促进急性炎症的消退。

• DOI: 10.4049/jimmunol.1900144
• 发表时间: 2019
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Zheng,Jing-Juan;PenaCalderin,Ernesto;Hill,BradfordG;Bhatnagar,Aruni;Hellmann,Jason
• 通讯作者: Hellmann,Jason

Omega-3 polyunsaturated fatty acids modify the inverse association between systemic inflammation and cardiovascular fitness.

• DOI: 10.1016/j.clnu.2021.02.006
• 发表时间: 2021-06
• 期刊: Clinical nutrition (Edinburgh, Scotland)
• 作者: Farley G;Riggs DW;Bhatnagar A;Hellmann J
• 通讯作者: Hellmann J

Cardiac-specific overexpression of aldehyde dehydrogenase 2 exacerbates cardiac remodeling in response to pressure overload.

• DOI: 10.1016/j.redox.2018.05.016
• 发表时间: 2018-07
• 期刊: Redox biology
• 影响因子: 11.4
• 作者: Dassanayaka S;Zheng Y;Gibb AA;Cummins TD;McNally LA;Brittian KR;Jagatheesan G;Audam TN;Long BW;Brainard RE;Jones SP;Hill BG
• 通讯作者: Hill BG

Paracrine signal emanating from stressed cardiomyocytes aggravates inflammatory microenvironment in diabetic cardiomyopathy.

• DOI: 10.1016/j.isci.2022.103973
• 发表时间: 2022-03-18
• 期刊: iScience
• 影响因子: 5.8
• 作者: Kaur N;Ruiz-Velasco A;Raja R;Howell G;Miller JM;Abouleisa RRE;Ou Q;Mace K;Hille SS;Frey N;Binder P;Smith CP;Fachim H;Soran H;Swanton E;Mohamed TMA;Müller OJ;Wang X;Chernoff J;Cartwright EJ;Liu W
• 通讯作者: Liu W