Core biochemistry equipment for antimicrobial peptide and nucleic acid aptamer research

抗菌肽及核酸适体研究核心生化设备

• 批准号: 345145-2007
• 负责人: DeRosa, Maria
• 金额: $ 7.83万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325952
• 关键词: Core; biochemistry; equipment; antimicrobial; peptide

The strategic research plan for Carleton University includes an enhanced focus on biomedical and biosciences research.  In keeping with this plan, the chemistry department has actively sought out researchers with interests at the interface of chemistry, biology, and medicine.  This is evidenced by the recent hires in the chemistry department in biophysical (Dr. Anatoli Ianoul) and bioinorganic (Dr. Maria DeRosa) chemistry.  This proposal is for the development of a core biochemistry facility to support these and other interdisciplinary research groups in the chemistry department at Carleton University.        Dr. Ianoul's research program is interested in developing a better understanding of antimicrobial peptides, considered to be potential alternatives to synthetic antibiotics.  Dr. DeRosa's research is focused on gaining a better insight into the unique properties of nucleic acid aptamers, to facilitate the use of these biological entities in biosensors and medical diagnostics.  Both these research programs have the potential to dramatically impact the health and well-being of Canadians.  In order to effectively pursue these areas of study, several key pieces of standard biochemistry equipment are urgently needed.  The equipment requested here includes: 1) a laboratory water purification system for providing DNase and RNase-free deionized water for biochemistry assays and experiments 2) an ultra-low temperature freezer (-86°C) for long-term storage of biological samples 3) an autoclave system for sterilization of equipment and liquid media, buffers, etc. 4) a shaking incubator for bacterial culture and 5) a biological safety cabinet for manipulations of bacteria for cloning and antimicrobial experiments.  A delay in the purchase of this equipment will restrict the ability of the DeRosa and Ianoul labs to perform the critical biochemistry experiments that will contribute to the timely impact of their research.  An investment in these basic pieces of equipment will enable the Ianoul and DeRosa laboratories to pursue their research endeavours at the boundary between chemistry, biology, and medicine, and will encourage the growth of interdisciplinary research and training in the chemistry department at Carleton University.

卡尔顿大学的战略研究计划包括加强对生物医学和生物科学研究的关注。根据这一计划，化学系积极寻找对化学，生物学，生物物理化学系最近的招聘证明了这一点（Anatoli Ianoul博士）和生物无机（Maria DeRosa博士）化学。该提案旨在开发一个核心生物化学设施，以支持卡尔顿大学化学系的这些和其他跨学科研究小组。 博士Ianoul博士的研究计划是对抗菌肽有更好的了解，抗菌肽被认为是合成抗生素的潜在替代品。DeRosa的研究重点是更好地了解核酸适体的独特性质，以促进这些生物实体在生物传感器和医疗诊断中的使用。这两个研究项目都有可能对加拿大人的健康和福祉产生巨大影响。为了有效地开展这些研究领域，迫切需要几个关键的标准生物化学设备。这里要求的设备包括：1）用于为生物化学测定和实验提供不含DNA酶和RNA酶的去离子水的实验室水净化系统86°C）用于生物样品的长期储存3）用于设备和液体介质，缓冲液，4）用于细菌培养的振荡培养箱和5）用于克隆和抗菌实验的细菌操作的生物安全柜。推迟购买这一设备将限制DeRosa和Ianoul实验室进行关键生物化学的能力。对这些基本设备的投资将使Ianoul和DeRosa实验室能够在化学，生物学和医学的边界上进行研究，并将鼓励卡尔顿大学化学系跨学科研究和培训的增长。