Spectrophotometric analysis system for determination of enzyme kinetics and metabolite concentrations

申请者的研究项目使用果蝇和鱼类来解决涉及复杂网络的分子遗传学、能量新陈代谢和生态生理学的问题。我们的研究项目各不相同，但通过集中关注酶功能和代谢复杂性，弥合了部门和学科的界限。虽然我们提出了不同的问题，但我们对酶动力学和代谢物浓度的准确量化有着共同的绝对要求。我们建议购买一套设备，用于对一系列酶活性和代谢物浓度进行光谱分析。我们建议购买一套设备，用于对一系列酶活性和代谢物浓度的大量样本进行光谱分析。该设备包建立在微天平和微板分光光度计的基础上，这两台设备是我们研究所需的。*该设备包在劳伦斯大学校园内将是独一无二的，允许进行其他不可能的研究项目。此外，该设备包将允许继续和扩大两个申请者实验室的研究计划，并允许在申请者以及化学、生物化学和生物系的其他研究人员的监督下培训本科生和研究生。

The applicants' research programs use fruit flies and fish to address questions involving the molecular genetics of complex networks, energy metabolism and ecological physiology.  Our research programs are distinct, but bridge departmental and discipline boundaries united by a central focus on enzyme function and metabolic complexity.  While we ask different questions, we share a common absolute requirement for the accurate quantification of enzyme kinetics and metabolite concentration, and fundamental need to efficiently and quickly process a large number of temperature-sensitive samples.  We propose to purchase a package of equipment necessary for spectrophotmetric assays of a large number of samples for a suite of enzyme activities and metabolite concentrations.  This package is built around a microbalance and a microplate spectrophotometer, two pieces of equipment that are required as a package for our research.  This package of equipment will be unique on the Laurentian University campus allowing research projects not otherwise possible.  Further, the package will allow continuation and expansion of the research programs of both laboratories of the applicants, and allow on the training of undergraduate and graduate students under the supervision of the applicants and other researchers in both Chemistry and Biochemistry and Biology Departments.