Cell culture facilities

要求细胞培养设施用于2个NSERC资助的研究项目和1个NSERC-CIHR合作健康研究项目，涉及生物化学，细胞信号传导，氧化应激，植物和分子生物学，细胞冷冻保存和环境毒理学，旨在了解：（1）细胞和分子对不同胁迫的反应（氧化应激、热休克、杀虫剂）（HeLa，A549，CHO，HepG 2）与植物细胞的关系;（2）调节植物低温耐受性的细胞、分子和遗传机制;（3）小麦蛋白对肝细胞、胰岛素分泌细胞INS 832/13和胰岛的保护作用机制。这些项目都是在哺乳动物细胞中进行的，需要每天全天使用细胞培养设施。Sanyo Incusafe CO2培养箱具有2个可堆叠的腔室，具有精确的CO2和温度控制以及污染控制的特点。单光束、双探测器红外（IR 2）CO2传感器提供精确的CO2控制和开门后的快速恢复。直接加热和空气夹套TM加热系统在微处理器PID控制下精确地调节温度。该培养箱具有三个特点，可降低细胞培养物中的污染风险：i）SterisonicTM H2 O2灭菌系统，可对整个培养室进行原位灭菌; ii）集成的SafeCellTM紫外线系统可清洁内部气流，以破坏空气中和潮湿的污染物; iii）InCu SaFeTM富铜不锈钢内表面可确保杀菌保护。Therapeuticine Jr Plus低温容器的储存容量为20箱，可容纳2000瓶细胞。带索引的样品架和样品盒系统可实现快速高效的样品检索。有一个超声波监测器用于液氮水平，以保护生物样品。必须在-196 ℃下储存冷冻细胞系，以控制传代次数和可能的细胞污染。在加拿大和国际层面，细胞培养设施对于这2名NSERC资助的高级研究人员的实验室中的高质量，有竞争力的研究和研究生培训是不可或缺的。

The Cell culture facilities are requested for 2 NSERC-funded research programs and 1 NSERC-CIHR Collaborative Health Research Project in biochemistry, cell signaling, oxidative stress, plant and molecular biology, cell cryopreservation and environmental toxicology, which aim to understand: (1) the cellular and molecular responses to different stresses (oxidative stress, heat shock, pesticides) in mammalian (HeLa, A549, CHO, HepG2) and plant cells; (2) the cellular, molecular, and genetic mechanisms that regulate low temperature tolerance in plants; and (3) mechanisms involved in the protective function of wheat proteins against cryopreservation damage in hepatocytes, insulin-secreting INS832/13 cells and pancreatic islets. These projects are all carried out in mammalian cells and require access to cell culture facilities on a fulltime and daily basis. The Sanyo Incusafe CO2 incubator with 2 stackable chambers has features of precision of CO2 and temperature control, and contamination control. The single-beam, dual detector infrared (IR2) CO2 sensor delivers precise CO2 control and quick recovery following door openings. The Direct Heat & Air JacketTM Heating System precisely regulates temperature under microprocessor PID control. The incubator has three features to decrease risk of contamination in cell cultures: i) A SterisonicTM H2O2 sterilization system for total chamber sterilization in situ; ii) An integrated SafeCellTM UV system cleans interior airflow to destroy airborne and humidity pan contaminants; iii) InCu SaFeTM copper-enriched stainless steel interior surfaces assure germicidal protection. The Thermolyne Locator Jr Plus cryogenic container has a storage capacity of 20 boxes and holds 2000 vials of cells. An indexed rack and box system allows quick and efficient sample retrieval. There is an ultrasound monitor for liquid nitrogen levels, to safeguard biological samples. It is essential to stock frozen cell lines at -196C to control passage number and possible cell contaminations. The cell culture facilities are indispensable for high quality, competitive research and graduate student training in the laboratories of these 2 senior NSERC-funded investigators, at the Canadian and international levels.