Cell culture facilities

细胞培养设施

• 批准号: 405926-2011
• 负责人: Averill(Bates), Diana
• 金额: $ 1.78万
• 依托单位: Université du Québec à Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472341
• 关键词: Cell; culture; facilities

The Cell culture facilities are requested for 2 NSERC-funded research programs and 1 NSERC-CIHR Collaborative Health Research Project in biochemistry, cell signaling, oxidative stress, plant and molecular biology, cell cryopreservation and environmental toxicology, which aim to understand: (1) the cellular and molecular responses to different stresses (oxidative stress, heat shock, pesticides) in mammalian (HeLa, A549, CHO, HepG2) and plant cells; (2) the cellular, molecular, and genetic mechanisms that regulate low temperature tolerance in plants; and (3) mechanisms involved in the protective function of wheat proteins against cryopreservation damage in hepatocytes, insulin-secreting INS832/13 cells and pancreatic islets. These projects are all carried out in mammalian cells and require access to cell culture facilities on a fulltime and daily basis. The Sanyo Incusafe CO2 incubator with 2 stackable chambers has features of precision of CO2 and temperature control, and contamination control. The single-beam, dual detector infrared (IR2) CO2 sensor delivers precise CO2 control and quick recovery following door openings. The Direct Heat & Air JacketTM Heating System precisely regulates temperature under microprocessor PID control. The incubator has three features to decrease risk of contamination in cell cultures: i) A SterisonicTM H2O2 sterilization system for total chamber sterilization in situ; ii) An integrated SafeCellTM UV system cleans interior airflow to destroy airborne and humidity pan contaminants; iii) InCu SaFeTM copper-enriched stainless steel interior surfaces assure germicidal protection. The Thermolyne Locator Jr Plus cryogenic container has a storage capacity of 20 boxes and holds 2000 vials of cells. An indexed rack and box system allows quick and efficient sample retrieval. There is an ultrasound monitor for liquid nitrogen levels, to safeguard biological samples. It is essential to stock frozen cell lines at -196C to control passage number and possible cell contaminations. The cell culture facilities are indispensable for high quality, competitive research and graduate student training in the laboratories of these 2 senior NSERC-funded investigators, at the Canadian and international levels.

在生物化学、细胞信号传导、氧化应激、植物和分子生物学、细胞低温保存和环境毒理学方面，nserc资助了2个细胞培养项目和1个NSERC-CIHR合作健康研究项目，旨在了解：(1)哺乳动物（HeLa、A549、CHO、HepG2）和植物细胞对不同应激（氧化应激、热休克、农药）的细胞和分子反应；(2)调控植物低温耐受性的细胞、分子和遗传机制；(3)小麦蛋白对肝细胞、分泌胰岛素的INS832/13细胞和胰岛抗低温保存损伤的保护作用机制。这些项目都是在哺乳动物细胞中进行的，需要全天和每天使用细胞培养设施。三洋Incusafe CO2培养箱采用2个可堆叠箱，具有CO2和温度控制精度高、污染控制好等特点。单光束，双探测器红外（IR2）二氧化碳传感器提供精确的二氧化碳控制和门打开后的快速恢复。直接加热和空气夹套加热系统在微处理器PID控制下精确调节温度。培养箱有三个特点，以减少污染的风险在细胞培养：1)sterisonic™H2O2灭菌系统，全室就地灭菌；ii)集成SafeCellTM UV系统清洁内部气流，破坏空气和湿度盘污染物；iii) InCu SaFeTM富铜不锈钢内表面确保杀菌保护。Thermolyne Locator Jr Plus低温容器的存储容量为20箱，可容纳2000瓶细胞。索引架和箱系统允许快速和有效的样品检索。有一个液氮水平的超声波监测器，以保护生物样本。为了控制传代数和可能的细胞污染，必须在-196℃下储存冷冻细胞系。这些细胞培养设施对于这两位nserc资助的高级研究员在加拿大和国际水平上的高质量、有竞争力的研究和研究生培训是必不可少的。