Acquisition of a 500 MHz High Resolution NMR Spectrometer

这项提案要求提供部分资金，用于购买一台500 MHz高分辨率核磁共振光谱仪，作为明尼苏达大学核磁共振用户的一个大社区共享设施。该仪器将用于研究生物分子在非水溶剂中的结构和稳定性，离子对DNA结构和相互作用的影响，BPTI、大肠杆菌硫氧还蛋白和通过基因工程获得的硫氧还蛋白类似物中的氢交换，硫氧还蛋白的结构测定，非血红素含铁蛋白的结构和机理，有机硫化合物的多肽合成和设计的新策略，表征木素衍生物的物理化学性质，以及完整细胞和组织中的细胞过程。除了促进上述研究，该仪器还将在明尼苏达大学的化学和生化研究项目中实现新的远程可能性和发展。

This proposal requests partial funding for the purchase of a 500 MHz High-Resolution NMR spectrometer to be used as a shared facility by a large community of NMR users at the University of Minnesota. The instrumentation will be used for research on the structure and stability of biological molecules in nonaqueous solvents, ion effects on the structure and interactions of DNA, hydrogen exchange in BPTI, E. coli thioredoxin and thioredoxin analogs obtained by genetic engineering, structure determination of thioredoxins, structure and mechanism of non-heme iron- containing proteins, new strategies for peptide synthesis and design of organosulfur compound, characterizing the physicochemical properties of lignin derivatives, and cellular processes in intact cells and tissues. In addition to facilitating the above research, the instrumentation will allow new long-range possibilities and developments in the chemical and biochemical research programs at the University of Minnesota.