Triple Quadrupole/SID and Pentaquadrupole Mass Spectrometers

这是一项探索表面诱导解离（SID）新方法在生物学问题上的适用性的建议。在本实验中，生物化合物将通过一种较新的解吸电离方法进行电离，并在串联质谱计的第一阶段选择感兴趣的电离化合物。然后，与固体表面的碰撞将用于解离选定的生物化合物，并通过扫描第二台分析仪记录SID子谱来推断其结构。该方法与传统串联质谱法相比的优势将通过获得一个具有离子/表面和气相碰撞能力的三重四极杆仪器来评估。新仪器将取代PI实验室的三重四极杆，提供更适合生物化合物研究的质量范围（4000 vs 1000道尔顿）。在评估SID的能力时，重点将放在肽测序上。还将讨论各种其他重要的生物学问题，包括DNA加合物与致癌碳氢化合物的分析，细胞壁低聚糖的结构，以及监测生物反应器中产生的产物。除了将SID用于生物化合物之外，还将探索对特定官能团具有选择性的离子/分子反应。新仪器的功能将提供给普渡大学的科学家，他们有特别重大的问题。利用将通过全校范围内的质谱设施和跨学科设施进行协调，以协调校园内质谱仪的使用和利用。

This is a proposal to explore the applicability of the new method of surface-induced dissociation (SID) to biological problems. In this experiment, biological compounds will be ionized by one of the newer desorption ionization methods and the ionized compound of interest selected in the first stage of a tandem mass spectrometer. Collisions with a solid surface will then be used to dissociate the selected biological compound and its structure will be deduced by recording an SID daughter spectrum by scanning the second analyzer. The advantages of this approach over conventional tandem mass spectrometry will be evaluated by acquiring a triple quadrupole instrument which incorporates capabilities for ion/surface as well as gas phase collisions. The new instrument will replace a triple quadrupole in the PI's laboratory, providing a mass range (4000 vs. 1000 daltons) better suited to the study of biological compounds. In evaluating the capabilities of SID, emphasis will be placed on peptide sequencing. A wide variety of other biologically important problems will also be addressed including the analysis of DNA adducts with carcinogenic hydrocarbons, structures of cell wall oligosaccharides, as well as monitoring products generated in bioreactors. In addition to the use of SID for biological compounds, ion/molecule reactions which are selective for particular functional groups will be explored. The capabilities of the new instrument will be made available to Purdue scientists who have particularly significant problems. Utilization will be coordinated through the Campus-wide Mass Spectrometry Facility, and interdisciplinary facility which coordinates access to and utilization of mass spectrometers on campus.