Engineering Research Equipment: Animal Cell Culture Facility

主要研究人员要求使用清洁蒸汽的高压灭菌器，这对于无菌培养必不可少，没有家庭蒸汽的有害残留物；两个生物安全帽，用于在使用转化细胞时保护工人；电子粒子计数器对于快速监测生长动力学和细胞大小至关重要。请求设备支持的项目示例包括：1)开发改进的生物反应器和从昆虫细胞-杆状病毒系统生产蛋白质和杀虫剂的操作策略；2)测试数学模型，预测细胞生理学对培养条件变化的反应；3)开发药物动力学模型的细胞培养类似物，以改进对潜在有毒化学品的风险评估；4)受体介导的细胞粘附动力学模型研究；5)利用转移细胞系建立细胞粘附血管的实验模型，以防止肿瘤细胞从体内的一个器官转移或扩散；6)开发用于培养附着依赖性细胞系的生物材料，以提高生物反应器的性能。

The Principal Investigators request an autoclave with clean steam, which is essential to aseptic culture without harmful residues from house steam; two biosafety hoods to protect workers when transformed cells are being used; and an electronic particle counter which is essential for rapidly monitoring growth kinetics and cell size. Examples of projects to be supported by the requested equipment are: 1) development of improved bioreactors and operating strategies for production of proteins and insecticides from the insect cell-baculovirus system; 2) testing of mathematical models that predict responses in cellular physiology to alterations in culture conditions; 3) development of cell-culture analogs to pharmokinetic models for the purpose of improved risk assessment with respect to potentially toxic chemicals; 4) model studies on the dynamics of receptor-mediated cell adhesion; 5) development ofan experimental model of cell adhesion to blood vessels to preventmetastasis or the spread of tumor cells from one organ within the body, using metastatic cell lines; and 6) the development of biomaterials for culture of attachment-dependent cell lines to improve bioreactor performance.