Silica Surface Modifications for High Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (HPCE)

该项目属于分析和表面化学的一般领域，以及分离科学的子领域。在这项为期三年的持续资助期间，Pesek教授和他的学生将致力于开发化学修饰程序，以改变用于高效液相色谱的多孔硅胶固定相和用于高效毛细管电泳法的玻璃毛细管壁。合成方法包括将表面硅醇官能团转化为氢化物，氢化物可以进一步通过硅氢化反应生成键合的有机部分。这些粘结材料与表面有直接的碳-硅键，而不是更常见的硅氧烷键，后者是由标准的有机硅烷反应过程产生的。在更广泛的分析溶液环境中，直接连接已被证明在层析和电泳上比硅氧烷连接更稳定。此外，还将进一步开发键合液晶固定相，并对其进行光谱、热和色谱表征。%这项研究建立在佩塞克博士之前的国家科学基金会拨款CHE-8814849下获得的结果的基础上，并将使流动相/固定相界面有更清晰的定义。此外，本研究还将为更合理、更高效地设计和合成液晶色谱固定相提供有见地的指导。

This project is in the general area of analytical and surface chemistry and in the subfield of separation science. During the tenure of this three-year continuing RUI grant, Professor Pesek and his students will pursue the development of chemical modification procedures for alteration of porous silica stationary phases for high performance liquid chromatography and glass capillary walls for high performance capillary electrophoresis. The synthetic approach involves converting surface silanol functionalities to hydrides which can be further reacted by hydrosilation reactions to produce bonded organic moieties. These bonded materials have a direct carbon-silicon bond to the surface instead of the more common siloxane linkage which arises from standard organosilane reactions procedures. The direct linkage has been proven both chromatographically and electrophoretically to be more stable than the siloxane linkages to a much wider range of analytical solution environments. Additionally, bonded liquid crystal stationary phases will be further developed and characterized spectroscopically, thermally, and chromatographically. %%% This research builds upon results obtained under Dr. Pesek's previous NSF grant, CHE-8814849, and will enable a clearer definition of the mobile phase/stationary phase interface. Additionally, this research should provide insightful guidance for the more rational and efficient design and synthesis of liquid crystal chromatographic stationary phases.