Acquisition of a 500 MHz NMR Spectrometer and Establishment of the Mississippi Resonance Facility

这份来自学术研究基础设施项目的合同将帮助密西西比州立大学化学系获得一台500兆赫光谱仪，用于研究。支持的研究活动包括对溶液中维生素B12衍生物构象的核磁共振研究，与维生素B12结合蛋白结合，与辅酶B12需要酶结合。还需要研究的是谷氨酰胺环转移酶的催化活性片段（5-10 kDa）在酶抑制剂存在和不存在的情况下的溶液构象；叶片叶绿体水分分布及环境参数对叶片叶绿体渗透调节的影响羧酸、氨基甲酸锡、n -烷基甲酰胺、烷基硫代甲酸酯、烷基硫代腈、n -叔丁基硝胺和n -叔丁基甲酰胺的构象和互变异构平衡；chloroheptafluorodirhenium二阶阴离子;通过1H、13C、17O和19F核磁共振谱测定顺式和反式环癸烯；苯胺吖啶类化合物可逆结合的几何和动力学以及光亲和探针共价结合的几何49个氨基酸残基神经肽、促肾上腺皮质激素释放因子的溶液结构及其构象变化。核磁共振（NMR）光谱是化学家用来阐明分子结构的最有力的工具。它用于识别未知物质，表征分子内原子的特定排列，并研究溶液中分子之间相互作用的动力学。获得最先进的核磁共振光谱是必不可少的化学家谁正在进行前沿研究。这些核磁共振研究的结果在聚合物和催化以及生物学等领域都很有用。

This award from the Academic Research Infrastructure Program will help the Department of Chemistry at Mississippi State University acquire a 500 MHz spectrometer which will be used in research. The research activity to be supported includes NMR studies of the conformation of derivatives of vitamin B12 in solution, bound to a vitamin B12 binding protein, and bound to a coenzyme B12 requiring enzyme. Also to be studied are the solution conformation of catalytically active fragments (5-10 kDa) of the enzyme glutamine cyclotransferase in the presence and absence of enzyme inhibitors; the distribution of leaf chloroplast water and the effects of environmental parameters on leaf chloroplast osmoregulation; conformation and tautomeric equilibria in carboxylic acids, tin carbamates, N-alkyl formamides, alkyl thioformates, alkyl thionitriles, N-tert-butylnitramine and N-tert-butylformamide; chloroheptafluorodirhenium dianion; cis-and trans-cyclodecene by 1H, 13C, 17O, and 19F NMR spectroscopy; the geometry and dynamics of the reversible binding of anilinoacridines and the geometry of the covalent binding of photo affinity probes; and the solution structure of the 49 amino acid residue neuropeptide, corticotropin-releasing factor and the conformational changes thereof. %%% Nuclear Magnetic Resonance (NMR) spectroscopy is the most powerful tool available to chemists for the elucidation of the structure of molecules. It is used to identify unknown substances, characterize specific arrangements of atoms within molecules, and to study the dynamics of interactions between molecules in solution. Access to state-of-the-art NMR spectrometry is essential to chemists who are carrying out frontier research. The results from these NMR studies are useful in areas such as polymers and catalysis, and in biology.