Fabrication of a Flow Cytometer Designed for the Synoptic Analysis of Marine Microbial Food Webs

马萨诸塞州理工学院将承担一项新的任务， 发展项目，以进一步推进使用流量 用于海洋科学研究目的的细胞仪。 流式细胞仪 最初是为生物医学研究团体开发的。 流式细胞仪已经发展成为生物学分析的有效工具。 海洋学研究，通过快速提供样本信息 含有小到海洋细菌的颗粒，大到单个的颗粒， 细胞浮游植物 这项工作将带来几个独特的 浮游生态系统流式细胞分析能力： 天气分析（光散射、丰度和自发荧光 直径从0.3到100微米的浮游生物）;详细 光散射信号的数字信号分析 先进的实时神经网络分析， 人口参数。 这些增强的能力将有助于 人口生态学家的研究过程， 遥感算法的发展。

The Massachusetts Institute of Technology will undertake an instru- mentation development project to further advance the use of flow cytometry for ocean science research purposes. Flow cytometers were originally developed for the biomedical research community. Flow cytometers have developed into effective tools for biological oceanographic research, by rapidly providing information on samples containing particles as small as marine bacteria to large single- celled phytoplankton. This work will bring several unique capabilities to flow cytometric analysis of pelagic ecosystems: synoptic analysis (light scatter, abundance, and autofluorescence of plankton ranging from 0.3 to 100 microns in diameter); detailed analysis of light scatter signals through digital signal processing; and advanced, real-time neural-network analysis of population parameters. These enhanced capabilities will facilitate process studies by population ecologists and they will contribute to the development of remote sensing algorithms.