Recombinant Production of Hyperthermophilic Enzymes: Effect of Temperature and Pressure

9421862 Clark该项目是研究利用重组DNA技术，通过在嗜温菌中表达这些酶，从极端微生物中生产酶。 其目的是使大量的这些酶的生物物理和生物化学表征，并最终酶的应用，而不需要培养极端微生物直接。 本研究的具体目标包括：（1）从极端嗜热菌Pyrococcus furiosus及其相关嗜热菌中克隆和鉴定基因序列;（2）在高压下在异源系统中表达基因，并在高压和高温下表征重组酶，以建立最佳的比活和组装;和（3）开发生物加工方法以获得足够量的酶用于结构测定和生物技术应用。 ***

9421862 Clark This project is on research to study the production of enzymes from extremophiles by expression of these enzymes in mesophiles, using recombinant DNA technology. The objective is to make available large quantities of these enzymes for biophysical and biochemical characterization and ultimately for enzymatic applications, without the need for culturing extremophiles directly. The specific objectives of this research include: (1) to clone and identify gene sequences from the hyperthermophile Pyrococcus furiosus and related thermophiles; (2) to express genes in heterologous systems at high pressure, and characterize recombinant enzymes under elevated pressure and temperature to establish optimal specific activity and assembly; and (3) to develop bioprocessing methods for obtaining enzymes in sufficient quantity for structural determination and biotechnological applications. ***