Acquisition of a 500 MHz NMR Spectrometer

我们建议购买一台三通道、脉冲梯度500 MHz的核磁共振波谱仪，用于来自康涅狄格学院和三一学院的8名卫斯理学院化学系、分子生物学和生物化学系以及外部用户的研究小组的博士和博士后研究培训。光谱学方面的研究生课程和指导，特别是核磁共振，是我们分子生物物理学项目的重点，由NIH培训基金支持。卫斯理学院还鼓励学生进行本科生论文研究，并计划让训练有素的本科生使用这一工具。卫斯理目前有两台400兆赫核磁共振仪器，其中一台最近进行了升级，另一台目前即将报废。300 MHz仪器支持化学本科教学实验室的需要和合成化学研究小组的常规分析需求。拟议的仪器将恢复我们进行高水平核磁共振研究的能力，并为蛋白质和核酸的2D和3D实验提供最先进的场强和电子设备，以及在较小分子上执行新实验的能力，这些实验在目前这里提供的核磁共振仪器上是不可行的。直接受益于这一倡议的卫斯理基础研究包括：a)由两个专门从事核磁共振光谱学研究的教员指导的项目：核酸和蛋白质的核磁共振研究(Bolton教授，化学，ACS，NSF和能源部资助)；RNA，DNA和蛋白质-DNA相互作用的核磁共振研究(Russu，MB&amp；B，NIH资助)；b)来自六个教员的项目，他们的工作涉及或与核磁共振光谱学有关：DNA核磁共振的理论方面(Beveridge教授，化学，NIH资助)；合成~阻滞剂的对映选择性催化(Bruno，化学，NSF资助)；研究领域包括：合成有机电化学(Fry教授、NSF教授和EPRI资助)；天然产物合成的核磁共振研究(Jacobi教授，NIH资助)；蛋白质-蛋白质和蛋白质-DNA相互作用的生物物理研究(Mukerji教授，MB&Amp；B，新教员)；~-内酰胺酶活性部位的结构和功能(Pratt教授，化学，NIH资助)；多电子/卤化物转移自交换反应动力学(Westmoland教授，化学，NIH资助)。此外，还有来自附近文理学院的两个教员的项目：带3蛋白结构域的解决方案(康涅狄格学院的布兰奇尼教授)和骨钙素及其相关多肽的核磁共振研究(普里戈迪奇教授，三一学院)，这两个项目都有外部资金。卫斯理科学项目的目标是为本科生和研究生提供高质量的课程和研究机会，为在科学和教育领域的职业生涯做准备。它提供了支持科学领域妇女的具体倡议，发展少数民族对科学的兴趣的方案，以及为残疾人提供机会。拟议的500 MHz核磁共振将为我们的Si7R机构和教育使命提供适当水平的科学仪器。

We propose the acquisition of a three channel, pulsed field gradient 500 MHz nuclear magnetic resonance spectrometer for use in doctoral and postdoctoral research training in research groups of eight Wesleyan faculty based in the Departments of Chemistry and the Department of Molecular Biology and Biochemistry and outside users from Connecticut College and Trinity College. Graduate courses and instruction in spectroscopy, and particularly NMR, are a focus of our Molecular Biophysics program, supported by an NIH Training Grant. Undergraduate thesis research is also encouraged at Wesleyan, and use of the instrument by trained undergraduates is also envisaged. There are currently two 400 MHz NMR instruments at Wesleyan, one of which has been recently upgraded and a second which currently is approaching obsolescence. A 300 MHz instrument supports the need of undergraduate teaching laboratories in chemistry and the routine analytical needs of the synthetic chemistry research groups. The proposed instrument would restore our capacity for high level NMR research and provide field strength and electronics for state of the art 2D and 3D experiments on proteins and nucleic acids, and also the ability to perform novel experiments on smaller molecules which are not feasible on the NMR instruments currently available here. Wesleyan based research to directly benefit from this initiative include a) projects directed by two faculty who specialize in NMR spectroscopy in their research: NMR Studies of Nucleic Acids and Proteins (Prof. Bolton, Chemistry, ACS, NSF and DOE funded); NMR Studies of RNA, DNA and Protein-DNA Interactions (Russu, MB&B, NIH funded); b) projects from six faculty whose work involves or relates to NMR spectroscopy: Theoretical Aspects of NMR on DNA (Prof. Beveridge, Chemistry, NIH funded); Enantioselective Catalysis in the Synthesis of ,~Blockers (Prof. Bruno, Chemistry, NSF funded); Synthetic Organic Electrochemistry (Prof. Fry, NSF and EPRI funded); NMR Studies in Natural Product Synthesis (Prof. Jacobi, Chemistry NIH funded); Biophysical Investigation of Protein-Protein and Protein-DNA Interactions (Prof. Mukerji, MB&B, a new faculty member); ,~-Lactamase Active Site Structure and Function (Prof. Pratt, Chemistry, NIH funded), Kinetics of Multielectron/Halide Transfer Self-Exchange Reactions (Prof. Westmoreland, Chemistry, NIH funded). In addition, there are projects from two faculty from nearby liberal arts colleges: Solution Structure of a Band 3 Protein Domain (Prof. Branchini, Connecticut College), and NMR Investigation of Osteocalcin and Related Peptides (Prof. Prigodich, Trinity College), both of whom have external funding. The objective of the science programs at Wesleyan is to provide high-quality courses and research opportunities for undergraduate and graduate students in preparation for careers in science and education. Specific initiatives in support of women in science, programs for developing the interest of minorities in science, and access for the handicapped are provided. The proposed 500 MHz NMR would place scientific instrumentation at an appropriate level for an institution of our Si7R and educational mission.