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Collaborative Research: Recombinant Production of Enzymes from Hypethermophiles: Effects of High Temperature and Pressure

Collaborative Research: Recombinant Production of Enzymes from Hypethermophiles: Effects of High Temperature and Pressure
合作研究:嗜热菌酶的重组生产:高温高压的影响
批准号:
9604561
负责人:
Douglas Clark
金额:
$39.2万
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-09-15 至 2001-08-31

项目摘要

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中文摘要
翻译
本提案旨在研究从嗜热微生物中开发酶和其他蛋白质的两个关键问题,即:(1)开发加工方法以获得大量这些酶,以供进一步研究和潜在应用;(2)研究极端嗜热微生物中压力诱导蛋白质稳定的机制,并设计出操纵这些酶的热稳定性的方法。位点定向诱变将以系统的方式使用,同时在高压下进行生物催化研究,以阐明压力对蛋白质热稳定性的影响以及这些多聚酶中亚基的相互作用。***
英文摘要
9604561 Clark This proposal is on research to examine two key issues in the development of enzymes and other proteins from thermophilic microorganisms which are to: (1) develop processing methods to obtain significant quantities of these enzymes for further investigation and potential application, and (2) examine the mechanisms of pressure-induced stabilization of proteins from extreme thermophiles and devise approaches to manipulate the thermostability of these enzymes. Site-directed mutagenesis is to be used in a systematic way, along with biocatalytic studies at elevated pressures to elucidate the effects of pressure on protein thermostability and the interaction of subunits in these multimeric enzymes. ***
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