Improving Mass Accuracy in FTICR Mass Spectrometry

该项目由Jon Amster教授和他的学生进行，并得到分析和表面化学的支持，涉及使用4.7特斯拉傅里叶变换质谱仪进行性能评估和分析方法开发。 该项目开发了一个完整的理解的空间电荷现象的FT ICR仪器的细胞，并记录其对质量测量精度的影响。 空间电荷现象是由一组新的复杂计算模型，通过改变池的物理设计和施加于其上的电势来进行实验。衍生离子被用作示例性应用以强调性能的改进。傅立叶变换离子回旋共振质谱仪的分析单元只能包含一定数量的离子在离子开始相互作用（空间电荷效应）之前。 空间电荷效应限制了仪器的质量测量精度。 格鲁吉亚大学的乔恩·阿姆斯特教授的这个项目在计算和实验中模拟了空间电荷效应，并通过开明的仪器设计，最大限度地减少了它对高电荷离子的影响。 其结果是大大提高了生物分子分析所需的质量测量精度。

This project, carried out by Professor Jon Amster and his students, and supported by Analytical and Surface Chemistry, involves performance evaluation and analytical methods development with a 4.7 Tesla Fourier Transform mass spectrometer. The project develops a complete understanding of the space charge phenomenon in the cell of an FT ICR instrument, and documents its effect on the mass measurement accuracy. The space charge phenomenon is modeled by a new set of sophisticated computations, and experiments are carried out by changes in the physical design of the cell and the potentials applied to it. High mass measurement accuracy for protein-derived ions is used as an exemplary application to emphasize improvements in performance.The analysis cell of a Fourier Transform ion cyclotron resonance mass spectrometer can only contain a certain number of ions before the ions begin to interact with each other (the space charge effect). The space charge effect limits the mass measurement accuracy of the instrument. This project of Professor Jon Amster of the University of Georgia models the space charge effect in calculation and experiment, and through enlightened instrument design, minimizes its effect on highly charged ions. The result is much improved mass measurement accuracy needed for biomolecular analysis.