Whole Genome Analysis of Pathogen-Host Recognition and Subsequent Responses in the Rice Blast Patho-System

稻瘟病是由稻瘟病真菌引起的，是水稻生产的主要制约因素，对全球粮食安全构成严重威胁。该项目的目标是通过对病原菌-寄主识别过程中发生的分子事件和随后的防御反应进行综合分析，阐明植物抗性的基础。我们的最终目标是设计出持久的抗病性。稻瘟病是进行这种分析的一个令人信服的实验系统。要完全了解这种相互作用需要了解病原体和宿主的基因。在这个项目中，将确定一套与水稻保护有关的关键无害环境技术。这些信息以及其他数据将用于功能基因组学研究，特别是微阵列分析和基因敲除，从病原体和宿主的角度揭示宿主-病原体识别的许多早期事件。因此，将为植物、细胞和病原体生物学研究界的分布和长期使用创造重要的新资源。为了方便获取资源和研究数据，将开发一个新的数据库（MGOS），作为植物和病原体研究界之间的桥梁。将以该研究为基础，为高中生设计和开发“基因组学与粮食安全”交互式教育组件。该项目包括一个主要组成部分，以促进来自代表性不足群体的本科生的研究经验，包括成立一个基因组俱乐部，该俱乐部将整合北卡罗莱纳州立大学的其他植物基因组项目。5万个具有相应表型信息、数码照片和基因组DNA的突变体。影响真菌渗透和致病机制的突变基因的鉴定和克隆。推测分泌的蛋白质、植物反应及其推断的功能。研究水稻寄主和真菌病原菌基因调控的微阵列和转录谱实验结果从感染水稻组织中提取SAGE文库。从感染和未感染的不同水稻组织中提取35000个est。公共数据库（MGOS），其中包含该项目过程中产生的所有数据。高中师生教学模块“基因组学与粮食安全”项目负责人，托马斯·米切尔，北卡罗来纳州立大学，thomas_mitchell@ncsu.edu, 2。项目主任，拉尔夫·迪恩，北卡罗莱纳州立大学，ralph_dean@ncsu.edu, 3。生物信息学，亚利桑那大学Cari Soderlund， cari@genome.arizona.edu4。教育和推广，Brenda Wojnowski，北卡罗莱纳州立大学，brenda_wojnowski@ncsu.edu项目信息的互联网地址（Web或电子邮件）：http://www.fungalgenomics.ncsu.edu2。http://www.riceblast.org3。thomas_mitchell@ncsu.edu4。ralph_dean@ncsu.edu

Rice blast disease, caused by the fungus Magnaporthe grisea, is a leading constraint to rice production and is a serious threat to food security worldwide. The goal of this project is to elucidate the basis of plant resistance through a comprehensive analysis of the molecular events that occur during pathogen-host recognition and the subsequent defense responses. Our ultimate goal is to design durable disease resistance. Rice blast is a compelling experimental system for conducting such analyses. A complete understanding of the interaction requires knowledge of the genes in both pathogen and host. In this project, a set of crucial ESTs involved in rice defense will be identified. This information, along with other data, will be used for functional genomics studies, specifically microarray analysis and gene knockout, to uncover many of the early events in host-pathogen recognition from the perspectives of both the pathogen and host. As a result, major new resources for distribution and long term use by the plant, cell and pathogen biology research communities will be created. To facilitate access to resources and research data, a new database (MGOS) will be developed that will serve as a bridge between plant and pathogen research communities. An interactive educational component "Genomics and Food Security" will be designed and developed for high school students founded on the research. The project includes a major component to promote research experiences for undergraduates from underrepresented groups including the formation of a Genomics Club that will integrate other Plant Genome projects at North Carolina State University.Deliverables:1. 50,000 mutants with corresponding phenotype information, digital photographs, and genomic DNA 2. Identification and cloning of mutated genes shown to affect fungal penetration and pathogenesis3. Putatively secreted proteins, plant responses, and their deduced functions4. Micro-arrays and the results of transcriptional profiling experiments looking at gene regulation in the rice host and the fungal pathogen5. SAGE libraries from infected rice tissue6. 35,000 ESTs from different rice tissues infected and non-infected7. Public database (MGOS) housing all data generated during the course of this project.8. Teaching module "Genomics and food security" for high school teachers and students Contact Information for Deliverables: 1. Project Leader, Thomas Mitchell, North Carolina State University, thomas_mitchell@ncsu.edu, 2. Project Director, Ralph Dean, North Carolina State University, ralph_dean@ncsu.edu, 3. Bioinformatics, Cari Soderlund, University of Arizona, cari@genome.arizona.edu4. Education and Outreach, Brenda Wojnowski, North Carolina State University, brenda_wojnowski@ncsu.edu Internet Addresses (Web or Email) for Project Information: 1. http://www.fungalgenomics.ncsu.edu2. http://www.riceblast.org3. thomas_mitchell@ncsu.edu4. ralph_dean@ncsu.edu