MRI: Acquisition of an Orbitrap Mass Spectrometer (MS) to Accelerate Studies on Small Molecules and Broaden MS Use at UCSC

在主要研究仪器项目（MRI）的资助和化学研究仪器项目（CRIF）的支持下，来自加州大学圣克鲁斯分校的Phillip Crews教授及其同事Rebecca Braslau、Theodore Holman、Roger Linington和Scott Lokey将获得一台轨道阱质谱仪。一般来说，质谱（MS）是用于鉴定和表征嵌入在复杂基质中的少量化学物质的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离成母离子和母离子的碎片离子，并测量它们的质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。在轨道仪器中，有一个充当电极的外部部分和一个同轴的内轴状电极，该电极在围绕内电极的轨道运动中捕获离子。电流的图像被产生，并使用数学（傅里叶变换）方法转换为质谱。这种分光计也将用于涉及学生的课程活动，其中许多学生来自代表性不足的群体。该提案旨在加强各级的研究和教育，特别是在以下领域：(a)研究代谢物和天然产物鉴定；(b)代谢组学;(c) lipidomics;(d)有机聚合物剖面。

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF), Professor Phillip Crews from the University of California Santa Cruz and colleagues Rebecca Braslau, Theodore Holman, Roger Linington and Scott Lokey will acquire an orbitrap mass spectrometer. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. In an orbitrap instrument there an outer section that acts as an electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the inner electrode. An image of the current is produced and converted to a mass spectrum using mathematical (Fourier transform) methods. This spectrometer will also be used in curricular activities involving students, many of whom are from underrepresented groups.The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) studying metabolites and natural product identification; (b) metabolomics; (c) lipidomics; and (d) organic polymer profiling.