MRI: Acquisition of an electrospray ionization triple quadrupole mass spectrometer with ion mobility spectrometry for improved plant lipidomics

堪萨斯州立大学获得了一台Sciex 6500+电喷雾电离三重四极杆质谱仪，该质谱仪带有线性离子陷阱、Selexion离子迁移率光谱、二元泵和用于样品导入的自动取样器，用于植物脂质的分析。该仪器将安装在堪萨斯脂质组学研究中心，这是堪萨斯州立大学的一个老牌分析实验室，进行基于质谱学的脂质分析。质谱仪的获得将直接影响和促进博士后、研究生和本科生的培养。职业生涯早期的教职员工和主要是本科院校的教职员工将受益于获得最先进的血脂分析。学习和使用质谱学的本科生将包括堪萨斯州立大学的学生，包括麦克奈尔的参与者和其他本科生学者？课程，以及来自历史上黑人的费耶特维尔州立大学。培训将包括为教职员工和受训人员举办的基于质谱学的脂类分析(脂组学)年度讲习班，以及侧重于分析和解释作物物种的脂组学数据的研究俱乐部。脂类组学尚未发挥其作为分析工具的最终潜力，因为有效地进行脂类分析，特别是植物脂类的方法、材料和计算资源并不方便。因此，堪萨斯脂肪组学研究中心的工作人员将通过提供咨询、样品制备方案、即插即用的质谱学方法、内部标准和数据处理系统来帮助希望在其机构实施脂质组学分析的科学家，从而产生适合上传到公共数据库并在其上进行分析的数据。通过使用收购的质谱仪获得的知识将用于改善食品、工业和主要农作物(包括高粱、山茶和小麦)的能源应用。收购的Sciex 6500+质谱仪及其附件将在保持高样品吞吐量的同时提供更高的脂类结构清晰度。与目前的技术相比，离子迁移率光谱附件将提高指定脂质结构特征的能力，而不会减缓分析工作流程。改进的脂类结构特异性将使科学家能够明确地将特定的脂类分配到代谢图中，将植物脂类的定量和结构信息直接与基因和酶联系起来，从而改变我们对模式植物和作物中脂类代谢途径的理解。质谱仪的收购将为来自15个实验室的研究人员的项目提供关键能力。此外，质谱仪将向新用户以及过去和现在的堪萨斯脂肪组学研究中心分析用户提供。项目将使用Sciex 6500+提供的高结构清晰度和离子迁移率光谱来准确地将脂体与植物基因组和物候组联系起来，同时研究脂类的作用以及脂类代谢和信号基因产物在植物中的功能。脂类将在全基因组关联研究和其他大规模的遗传变异植物种群研究中进行分析，以确定在植物胁迫反应中可能在脂类代谢和信号传递中发挥作用的候选基因。其他项目将从生物化学和功能上描述植物对压力的反应以及生长和发育过程中发生的脂肪代谢事件。新仪器还将推进科学家修改植物种子油成分以提高产量和质量的工作。因此，获得的质谱仪将促进食品、工业和能源作物的改良。

An award is made to Kansas State University to acquire a Sciex 6500+ electrospray ionization triple quadrupole mass spectrometer with a linear ion trap, SelexION ion mobility spectrometry, a binary pump, and an autosampler for sample introduction, to be used in analysis of lipids from plants. The instrument is to be housed at the Kansas Lipidomics Research Center, an established analytical laboratory performing mass spectrometry-based lipid analysis at Kansas State University. The mass spectrometer acquisition will directly impact and advance the training of postdoctoral trainees, graduate students, and undergraduates. Early-career faculty and faculty at primarily undergraduate institutions will benefit from access to state-of-the-art lipid analysis. Undergraduates learning and using mass spectrometry will include students at Kansas State University, including participants in the McNair and other undergraduate scholars? programs, and from historically black Fayetteville State University. Training will include an annual workshop on mass spectrometry-based lipid analysis (lipidomics) for faculty and trainees and a research club focused on analysis and interpretation of lipidomics data on crop species. Lipidomics has not reached its ultimate potential as an analytical tool, because the methods, materials, and computational resources to effectively carry out lipid profiling, particularly for plant lipids, are not conveniently available. Thus, Kansas Lipidomics Research Center personnel will aid scientists who wish to implement lipidomics analyses at their institutions by providing consultation, protocols for sample preparation, plug-and-play mass spectral methods, internal standards, and data processing systems, resulting in data suitable for upload to and analysis at a publicly available database. Knowledge obtained through use of the acquired mass spectrometer will be applied to improve food, industry, and energy applications in major crops including sorghum, camelina, and wheat.The acquired Sciex 6500+ mass spectrometer with accessories will provide improved definition of lipid structure while maintaining high sample throughput. The ion mobility spectrometry accessory will improve ability to specify lipid structural features without slowing the analytical work flow, compared to current technologies. The improved lipid structural specificity will provide scientists with the ability to unambiguously assign specific lipids to metabolic maps, connecting quantitative and structural information on plant lipids directly with genes and enzymes, thus transforming our understanding of lipid metabolic pathways in model and crop plants. The mass spectrometer acquisition will deliver critical capabilities for the projects of researchers from fifteen laboratories. Additionally, the mass spectrometer will be made available to new users as well as past and current Kansas Lipidomics Research Center analytical users. Projects will use the high structural definition provided by the Sciex 6500+ with ion mobility spectrometry to link the lipidome accurately with plant genomes and phenomes, while investigating the roles of lipids and the functions of lipid metabolizing and signaling gene products in plants. Lipids will be analyzed in genome wide association studies, and other large-scale studies of genetically variant plant populations, identifying candidate genes putatively functioning in lipid metabolism and signaling during plant stress responses. Other projects will biochemically and functionally characterize lipid metabolic events that occur during plant responses to stress and during growth and development. The new instrument also will advance the work of scientists modifying plant seed oil composition for increased yield and quality. Thus, the acquired mass spectrometer will facilitate food, industrial, and energy crop improvement.