MRI: Acquisition of an electrospray ionization triple quadrupole mass spectrometer with ion mobility spectrometry for improved plant lipidomics

堪萨斯州立大学获得Sciex 6500+电喷雾电离三重四极杆质谱仪，该质谱仪具有线性离子阱，SelexION离子迁移谱，二元泵和用于样品导入的自动进样器，用于分析植物脂质。该仪器将被安置在堪萨斯州脂质组学研究中心，这是堪萨斯州立大学一个建立的分析实验室，进行基于质谱的脂质分析。质谱仪的获取将直接影响和推进博士后、研究生和本科生的培养。初级职业教师和主要本科院校的教师将受益于最先进的脂质分析。学习和使用质谱的本科生将包括堪萨斯州立大学的学生，包括麦克奈尔项目的参与者和其他本科生学者？以及历史上的黑人费耶特维尔州立大学。培训将包括为教师和学员举办的基于质谱的脂质分析（脂质组学）年度研讨会，以及一个专注于分析和解释作物物种脂质组学数据的研究俱乐部。脂质组学还没有达到其作为分析工具的最终潜力，因为有效进行脂质分析的方法、材料和计算资源，特别是植物脂质分析，都不方便获得。因此，堪萨斯脂质组学研究中心的工作人员将通过提供咨询、样品制备协议、即插即用质谱方法、内部标准和数据处理系统，帮助希望在其机构中实施脂质组学分析的科学家，从而使数据适合上传到公开可用的数据库并进行分析。通过使用所获得的质谱仪获得的知识将用于改善主要作物的食品、工业和能源应用，包括高粱、亚麻荠和小麦。获得的Sciex 6500+质谱仪及其附件将提供更好的脂质结构清晰度，同时保持高样品通量。与目前的技术相比，离子迁移率光谱附件将提高指定脂质结构特征的能力，而不会减慢分析工作流程。改进的脂质结构特异性将为科学家提供明确地将特定脂质分配到代谢图谱的能力，将植物脂质的定量和结构信息直接与基因和酶联系起来，从而改变我们对模型和作物植物脂质代谢途径的理解。质谱仪的获取将为来自15个实验室的研究人员的项目提供关键能力。此外，质谱仪将提供给新用户以及过去和现在的堪萨斯脂质组学研究中心的分析用户。项目将利用Sciex 6500+提供的高结构清晰度和离子迁移谱技术，准确地将脂质组与植物基因组和现象联系起来，同时研究脂质在植物中的作用以及脂质代谢和信号基因产物的功能。脂质将在全基因组关联研究和其他遗传变异植物群体的大规模研究中进行分析，确定在植物应激反应中脂质代谢和信号传导中推测起作用的候选基因。其他项目将从生物化学和功能上描述植物对胁迫和生长发育过程中发生的脂质代谢事件。新仪器还将推进科学家们修改植物种子油成分以提高产量和质量的工作。因此，获得的质谱仪将促进食品、工业和能源作物的改进。

An award is made to Kansas State University to acquire a Sciex 6500+ electrospray ionization triple quadrupole mass spectrometer with a linear ion trap, SelexION ion mobility spectrometry, a binary pump, and an autosampler for sample introduction, to be used in analysis of lipids from plants. The instrument is to be housed at the Kansas Lipidomics Research Center, an established analytical laboratory performing mass spectrometry-based lipid analysis at Kansas State University. The mass spectrometer acquisition will directly impact and advance the training of postdoctoral trainees, graduate students, and undergraduates. Early-career faculty and faculty at primarily undergraduate institutions will benefit from access to state-of-the-art lipid analysis. Undergraduates learning and using mass spectrometry will include students at Kansas State University, including participants in the McNair and other undergraduate scholars? programs, and from historically black Fayetteville State University. Training will include an annual workshop on mass spectrometry-based lipid analysis (lipidomics) for faculty and trainees and a research club focused on analysis and interpretation of lipidomics data on crop species. Lipidomics has not reached its ultimate potential as an analytical tool, because the methods, materials, and computational resources to effectively carry out lipid profiling, particularly for plant lipids, are not conveniently available. Thus, Kansas Lipidomics Research Center personnel will aid scientists who wish to implement lipidomics analyses at their institutions by providing consultation, protocols for sample preparation, plug-and-play mass spectral methods, internal standards, and data processing systems, resulting in data suitable for upload to and analysis at a publicly available database. Knowledge obtained through use of the acquired mass spectrometer will be applied to improve food, industry, and energy applications in major crops including sorghum, camelina, and wheat.The acquired Sciex 6500+ mass spectrometer with accessories will provide improved definition of lipid structure while maintaining high sample throughput. The ion mobility spectrometry accessory will improve ability to specify lipid structural features without slowing the analytical work flow, compared to current technologies. The improved lipid structural specificity will provide scientists with the ability to unambiguously assign specific lipids to metabolic maps, connecting quantitative and structural information on plant lipids directly with genes and enzymes, thus transforming our understanding of lipid metabolic pathways in model and crop plants. The mass spectrometer acquisition will deliver critical capabilities for the projects of researchers from fifteen laboratories. Additionally, the mass spectrometer will be made available to new users as well as past and current Kansas Lipidomics Research Center analytical users. Projects will use the high structural definition provided by the Sciex 6500+ with ion mobility spectrometry to link the lipidome accurately with plant genomes and phenomes, while investigating the roles of lipids and the functions of lipid metabolizing and signaling gene products in plants. Lipids will be analyzed in genome wide association studies, and other large-scale studies of genetically variant plant populations, identifying candidate genes putatively functioning in lipid metabolism and signaling during plant stress responses. Other projects will biochemically and functionally characterize lipid metabolic events that occur during plant responses to stress and during growth and development. The new instrument also will advance the work of scientists modifying plant seed oil composition for increased yield and quality. Thus, the acquired mass spectrometer will facilitate food, industrial, and energy crop improvement.