多様な生体医学応用のためのインテリジェント画像活性化細胞選抜法　の応用展開

(a) iIACS机器得到了改进，在系统性能上远远超过了以前最先进的iIACS机器，扩大了应用和发现的范围。其通量和灵敏度是现有系统的20倍。(b)通过采用VIFFI显微镜，iIACS机器具有更高的成像性能，有望在免疫学、微生物学、干细胞生物学、癌症生物学、病理学和合成生物学等领域得到广泛应用。iIACS机器的成像性能通过各种细胞类型进行验证，如Jurkat细胞的FISH图像，芽殖酵母细胞的成像和分选。(c)我开发了一种基于粘弹性流体的三维聚焦器，并将其用于单粒子拉曼分析，以及VIFFI平台的流动模块，可以实现高通量的单细胞成像和分析，并且稳定性更高。

(a) The iIACS machine has been improved, which far surpasses the previous state-of-the-art iIACS machine in system performance, expanding the range of applications and discoveries. Its throughput and sensitivity are 20 times superior to those achieved in previous system.(b) By employing the VIFFI microscope, the iIACS machine has higher imaging performance, holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology. The imaging performance of the iIACS machine was validated using various cell types, like the FISH images of the Jurkat cells, imaging and sorting for the budding yeast cells.(c) I have developed a viscoelastic fluid-based 3D focuser, and employed it for single-particle Raman analysis, as well as into the flow module in VIFFI platform, which can enable high-throughput single-cell imaging and analysis with higher stability.