Development of universal transcriptome analysis method toward functional genomics in woody plants

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To advance the functional genomics of non-model woody plant, Fagus crenata (Fagaceae), an ecologically important tree species, two studies was conducted as follows; (1) DNA microarray created from a F. crenata cDNA sequence data-set is examined if versatile tool to study transcriptome comparing among intraspecific variations or not, and (2) F. crenata draft genome was generated. The transcriptome analysis by using the F. crenata DNA microarray was available for three populations, Kuromatsunai in Hokkaido Island, Tanzawa and Fuji in Honshu Island, suggesting the method is versatile in F. crenata. A draft genome of F. crenata was generated with 442 Mbp (including gap), allowing 48,618 predicted genes. This genomic information of F. crenata would contribute to accelerate the functional genomics of woody plants in family Fagaceae.