Analysis of uniparental elimination of chromosomes in wide crosses

在一些种间杂交植物中发生了完全的单亲体染色体消除。本项目的目的是研究杂交胚胎发育过程中父本染色体的选择性消除过程，并确定高度保守的组蛋白H3样着丝粒蛋白A （CENH3）是否作为有丝分裂依赖性消除过程的调节因子。此外，参与有丝分裂独立的染色体消除过程的基因将被确定。不同类型的黄顶花杂交将被用作模型，因为在这种物种组合中，染色体保留的程度受到双亲基因型和受精后环境条件的影响。除了更好地了解新形成的杂交种中亲本基因组的相互作用外，所获得的知识还可以建立产生单倍体或新物种组合的有效方法。

Complete uniparental chromosome elimination occurs in some interspecific hybrids of plants. The aim of this project is to investigate the processes of selective elimination of paternal chromosomes during the development of hybrid embryos and to establish whether the highly conserved histone H3 like centromeric protein A (CENH3) acts as a regulator of the mitosis-dependent elimination process. In addition, genes involved in the mitosis-independent chromosome elimination process will be identified. Different types of H. vulgare x H. bulbosum crosses will be used as a model, because in this species combination the degree of chromosome retention is influenced by the genotype of both parents as well as environmental conditions after fertilization. Besides a better understanding on interaction of parental genomes in newly formed hybrids, the gained knowledge could result in establishing efficient methods for generating of haploids or new species combinations.