Development of X-ray-and Chemi-luminescence Video-Microscopy and its Application to the Analysis of Biodynamics in Micro Region

1)化学发光视频显微镜的发展通过光子计数摄像机和光学显微镜的组合，开发了光敏视频显微镜，可以方便地用于微观区域生物系统动态方面的观察。2)化学发光视频显微镜的应用本发明的系统用于观察嗜中性粒细胞的吞噬作用，其中产生超氧化物以杀死外来颗粒。该超氧化物与发光体发生化学发光反应。成功地分析了该生成过程的时间过程和超氧化物的量，并显示了该方法未来的成果潜力。3) x射线发光现象用同步加速器发射的强x射线，测量了其发光强度，其发光强度与溶剂的疏水性有关，在水溶液中较弱。该现象可用于探针周围疏水性的监测。4)视频分析系统在其他动态方面的应用本系统应用于液相色谱分离过程的分析。该系统直接观察了色谱柱中的带分离过程，清除了所有色谱师想象不到的真实分离过程。实验结果表明，所开发的视频分析系统在微观和宏观领域的动态分析方面都具有丰富的潜力。

1)Development of Chemiluminescence Video MicroscopyBy assembly of a photon-counting video-camera and optical microscopy, photosensitive video-microscopy was developed which can be applied easily to the observation of dynamic aspects of biological systems in micro region.2)Application of this Chemiluminescence Video MicroscopyThe developed system was applied to the observation of phagocytosis of Neutrophils in which superoxide is generated to kill the foreign particles. This superoxide was detected as the chemiluminescence with reaction of luminor. The time course of this generation process and the amount of superoxide were successfully analyzed and showed future fruitful potential of this method.3)X-ray Luminescent PhenomenaIrradiating the strong X-ray from synchrotron, the intensity of luminescence was measured and the intensity depends on the hydrophobisity of solvent, and was weak in aqueous solvent. This phenomena can be applied to the monitoring of hydrophobisity of probe surrounding.4)Application of Video Analyzing System to Other Dynamic AspectsThis system was applied to the analysis of separation process of Liquid Chromatography. The band separation process in the column was directly observed by this system and the real separation process was cleared that has been only under imagination of all chromatographer.It was shown that the developed video-analyzing system has fruitful potentials for the analysis of dynamic aspects in not only micro-region but also in macro-region.