Development of New Packing Materials in Chromatography Based on Molecular Recognition Concept

本项目介绍了三种基本方法来提出、设计、合成和评价一种新型固定相，该固定相对多环芳烃（PAHs）和富勒烯的分离具有良好的分辨率和选择性。这些步骤是基于分子识别机制。对于多环芳烃，溶质是二维分子或立体大分子。首先，我们对现有的用于分离多环芳烃和富勒烯的ODS固定相进行了评价，为我们设计新的固定相以提高选择性和性能提供了基本的认识和知识。其次，评价了不同于ODS相的固定相。在此评价中，合成了各种类型的相，并对LC性能进行了测试。对其保留机理有了许多新的认识。利用这些实验得到的信息，可以用计算机能量计算和图形表示来设计新的固定相。然后通过有机反应设计合成新的相，以理解ODS和其他固定相得到的实验结果。在确认这些结果后，多环芳烃和富勒烯分离的选择性增强被看到，一个可以多环芳烃富勒烯。这些新相为多腿苯基键合相和C60键合相，均为该领域的新相。该项目已进入评价新设计和合成的用于多环芳烃和富勒烯分离的相的阶段。结果清楚地表明，基本分子识别机制对提高溶质分离的选择性起着很好的作用。该方法可以显示出针对特定分离问题设计定制固定相的未来趋势，该项目在未来的分离科学中具有重要的价值。

In this project, three basic approaches are introduced to propose, design, synthesize and evaluate a novel stationary phase which can offer excellent resolution and selectivity for the separations of polycyclic aromatic hydrocarbons (PAHs) and fullerenes. The steps are based on the molecular recognition mechanism. For PAHs the solutes are two dimensional molecules or steric bulky molecules. Firstly we have evaluated easily available ODS stationary phases for the separation of PAHs and fullerenes and the results provided us with the basic understanding and knowledge for designing novel stationary phases for enhancing selectivity and performance. Secondly stationary phases different from ODS phases were evaluated. In this evaluation various types of phases were synthesized and LC performance has been examined. Many new information about the retention mechanism have been obtained. With this experimentally obtained information novel stationary phases can be designed using computer energy calculations and graphical presentation. And then new phases designed were synthesized by organic reactions in order to understand the experimental results obtained by ODS and other stationary phases. After th confirmation of those results in which selectivity enhancements for PAHs and fullerenes separations are seen, one could PAHs fullerenes. Those new phases were multilegged phenyl group bonded phases and C60 bonded phases which are both new phases in this field. The project has entered into the evaluation of those newly designed and synthesized phases for PAHs and fullerenes separations. The results have clearly indicated the basic molecular recognition mechanism is working very well for the selectivity enhancements of those solutes separations. This approach can show the future trends in designing the tailored stationary phases for the particular separation problems and the value of thisproject is very important in the future separation sciences.