A study on long-term and continuous observation and analysis of microcirculation under artificial circulation

本研究的目的是利用CCD完成微循环观察的微型探头，并分析人工循环对微循环的影响，特别是其流态对微循环的影响。1）设计了直径4.2 mm、长4 mm、放大倍数3倍的非球面伦茨，用丙烯酸树脂制成。2）将伦茨与CCD单元用环氧树脂模压制成微循环观察用微探针。活体组织的焦点能够通过转动透明台来安装组织来调整。3）将探针植入山羊体内。在调整其焦点后，将皮下结缔组织安装并固定在台上。将微循环图像无线传输到电视上。4）连续观察微循环24 h。实验证明，该系统能清晰地观察到红细胞和白细胞的行为。然而，与此同时，由于山羊的身体运动，有时会使移植的组织失去焦点。作为微循环的另一种评价方法，拍摄了球结膜微动脉和微静脉的照片。6）对1只山羊植入波动泵式人工心脏后球结膜微循环进行了53天的观察。说明该分析方法是一种较好的微循环评价方法。

The objective of this study is to complete a micro probe to observe microcirculation that have been developing by our group using CCD and to analyze the influemce of artificial circulation, especially its flow pattern on microcirculation. The following results have been obtained.1) Aspherical lenz having 4.2 mm in diameter, 4 mm long and 3 times magnification, was designed and was made of acrylic resin.2) A micro probe for observation of microcirculation was made by molding the lenz with a CCD unit using epoxy resin. The focus of the living tissue was able to adjust by turning a tranparent stage to mount the tissue.3) The probe was implanted into a goat. A subcutaneous connective tissue was mounted and fixed on the stage after adjustment of its focus. The microcirculation image was sent to a TV wirelessly.4) The microcirculation was observed continuously for 24 hours. It was proved that the behavior of erythrocyte and leukocyte could be observed clearly. Howevere, at the same moment, it became a problem that the mouted tissue was sometimes out of focus by the body movement of the goat. A remote focus mechanism is now designing as the next turget.5) As the other evaluation method of microcirculation, arteriole and venule of bulb conjunctiva was taken in a photograph. The diameter of arteriole and venule abd their ratio was analysed.6) The microcirculation of bulb conjunctiva of a goat implanted a undulation pump total artificial heart was observed for 53 days. It was clarified that this analysis method was a good evaluation method of microcirculation.