Development of a risk assessment method for dental materials using a bone metabolic system

释放到环境中的许多化学物质被认为会扰乱人类和动物的正常内分泌功能。这些内分泌干扰物(EDCs)会影响生殖健康和发育。目前，人们的注意力集中在体内和体外筛选策略的开发上，以鉴定和分类内皮细胞，以确定此类化学物质是否对人类健康构成危害。在以前的研究中，我们报道了化合物的雌激素活性，受体结合分析，以及使用培养细胞和酵母细胞的受体基因表达分析，以及酵母双杂交试验。这些筛选试验被应用于口腔材料的风险评估。尽管这些方法在检测雌激素活性方面是有用的，但它们也有其局限性。特别是，人们对内皮样细胞是否影响骨形成知之甚少。本研究探讨了牙科材料的遗传毒性和雌激素效应。人们希望利用成骨细胞建立体内EDCs和遗传毒性化学物质的生物检测方法，然后应用于判断口腔中从牙科材料中渗出的化学物质的安全性。总结这些研究中进行的各种分析的结果，得出以下结论：1)树脂单体和增塑剂等化学物质在聚合后从牙科树脂中渗出。2)牙科材料的某些成分具有遗传毒性或雌激素样活性。3)检测暴露于化学品的成骨细胞中RANKL、OPG、ERα和ERβ的表达有助于评估骨代谢系统的风险评估。

Many chemicals released into the environment are believed to disrupt normal endocrine functions in humans and anima1s. These endocrine-disrupting chemicals (EDCs) affect reproductive health and development. Currently, attention is focused on the development of in vivo and in vitro screening strategies to identify and classify EDCs, in order to determine whether such chemicals pose a hazard to human health. In previous studies, we reported on the estrogenic activity of compounds, receptor-binding assays, and receptor gene expression assays using cultured cells and yeast cells, and a yeast two-hybrid assay. These screening tests were applied to the risk evaluation of dental materials. Despite the usefulness of these methods for detecting estrogenicity, they have their limitations. In particular, very little is known about whether EDCs affect bone formation. This study investigated the effects of the genotoxicity and estrogenicity of dental materials. It is hoped that a bioassay of EDCs and genotoxic chemicals in vivo can be established, using osteoblasts, and then applied, to judge the safety of the chemicals that leach from dental materials in the mouth. Summarizing the results of the various analyses carried out in these studies, the following conclusions were drawn:1) Chemicals such as resin monomers and plasticizers leach out of dental resins after polymerization.2) Some components of dental materials have genotoxic or estrogenic activity.3) Measurements of RANKL, OPG, ERα, and ERβ mRNA in osteoblasts exposed to chemicals were useful for evaluating risk assessment in a bone metabolic system.