Miniaturized ultra-high performance supercritical fluid chromatography with chip-compatible flame ionization detector and ion mobility spectrometer

从初步工作中得知，超临界流体色谱（SFC）的小型化允许以无与伦比的高速分析对映体。然而，目前还没有科学的方法可以a)在实践中达到理论上可能的分离性能，b)以足够快的速度和高灵敏度检测洗脱物质。通过基于模块化微流体（芯片-SFC与微流控背压控制）和与高灵敏度和芯片兼容检测器的无死体积耦合的有针对性的方法，我们的目标是理论上可能的SFC分离性能。将开发具有直接微流控耦合的紧凑型火焰电离检测器和具有x射线电离的双离子迁移谱仪，以及为快速芯片sfc定制的具有流控聚焦的电离区。除了极其灵敏的检测洗脱物质，离子迁移率光谱法允许一个正交分析的离子迁移率。在Belder （Leipzig）和Zimmermann （Hannover）两个工作组的联合研究项目中，将为首次实现小型化SFC分析系统奠定科学基础，该系统具有相当大的应用潜力，特别是在异构体的高速分析方面。

As known from preliminary work, miniaturization of supercritical fluid chromatography (SFC) allows for analyzing of enantiomers at unsurpassed high speed. However, no scientific approach currently exists to a) reach the theoretically possible separation performance in practice and b) detect the eluted substances sufficiently fast and with high sensitivity. With a targeted approach based on modular microfluidics (chip-SFC with microfluidic backpressure control) and a dead volume-free coupling to highly sensitive and chip-compatible detectors, we are aiming for the theoretically possible separation performance of SFC. Therefore, a compact flame ionization detector with direct microfluidic coupling and a dual ion mobility spectrometer with X-ray ionization and an ionization region tailored to fast chip-SFC with fluidic focusing of the eluate will be developed. Besides extremely sensitive detection of the eluted substances, ion mobility spectrometry allows for an orthogonal analysis by ion mobility. In this joint research project of the two working groups Belder (Leipzig) and Zimmermann (Hannover), the scientific basis for the first realization of a miniaturized SFC analysis system will be laid, which has considerable application potential, especially in high-speed analysis of isomers.