Studies on Bilirubin Excretion Mechanism of Fish

1. 建立了高效液相色谱法分析鱼类胆色素的方法。该方法快速、灵敏、可靠，似乎可用于测定鱼类胆汁和其他组织中的胆汁色素。采用高效液相色谱法分析了牛磺酸伴苯肼致溶血性贫血黄尾鱼胆汁中胆色素含量的变化。结果表明，牛磺酸在黄尾鱼胆红素排泄机制中起重要作用。对黄疸黄尾鱼的TBA值、α -生育酚和胆汁色素含量进行了分析。同时对肝、脾进行组织学观察。结果证实肝内脂肪酸的过氧化有助于黄疸的发生。通过高效液相色谱法测定鳗鱼胆汁中胆红素- ix β异构体的含量，并分析其游离酸及其甲酰苯甲酸乙酯重氮衍生物。胆红素ix β占胆汁中总胆红素的30- 40%。对黄疸鳗鱼肝脏进行了组织病理学和酶免疫化学观察。用高效液相色谱法测定血浆中胆红素含量。结果证实，内分泌系统的功能障碍有助于该病的发生。

1. HPLC method for analysis of bile pigment in fish was constructed. The method is rapid, sensitive, and reliable and seems to be available for measurement of bile pigments in the bile and other tissues of fish.2. Changes in bile pigment contents in the bile of yellowtail fed on taurine with phenylhydrazine induced hemolytic anemia were analyzed by HPLC. The results indicate that taurine plays an important role for bilirubin excretion mechanism of yellowtail.3. TBA values, content of alpha-tocopherol and bile pigments in jaundiced yellowtail were analyzed. The liver and spleen were also observed histologically. The results confirm that the peroxidation of fatty acids in the liver contributes the occurrence of the jaundice.4. Bilirubin-IX beta isomer was detected in the bile of eel, as evidenced by HPLC analysis of free acid and its ethyl anthranilate diazo derivatives. Bilirubin-IX beta accounted for 30-40 % of the total bilirubins in the bile.5. The liver of jaundiced eel was observed histopathologically and enzyme-imunochemically. Bilirubin contents in the plasma were also analyzed by HPLC. The results confirm that the dysfunction of the endocrine systems contributes the occurrence of the disease.